Zimmermann A, Reimmann C, Galimand M, Haas D
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Mol Microbiol. 1991 Jun;5(6):1483-90. doi: 10.1111/j.1365-2958.1991.tb00794.x.
Anaerobic growth of Pseudomonas aeruginosa on nitrate or arginine requires the anr gene, which codes for a positive control element (ANR) capable of functionally complementing an fnr mutation in Escherichia coli. The anr gene was sequenced; it showed 51% identity with the fnr gene at the amino acid sequence level. Four cysteine residues known to be essential in the FNR protein are conserved in ANR. The anr gene product (deduced Mr 27,129) was visualized by the maxicell method and migrated like a 32 kDa protein in gel electrophoresis under denaturing conditions. An anr mutant of P. aeruginosa constructed by gene replacement was defective in nitrate respiration, arginine deiminase activity, and hydrogen cyanide biosynthesis, underscoring the diverse metabolic functions of ANR during oxygen limitation. Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas syringae, and Pseudomonas mendocina all had a functional analogue of ANR, indicating that similar anaerobic control mechanisms exist in these bacteria.
铜绿假单胞菌在硝酸盐或精氨酸上的厌氧生长需要anr基因，该基因编码一种能够在功能上互补大肠杆菌中fnr突变的正调控元件（ANR）。对anr基因进行了测序；它在氨基酸序列水平上与fnr基因显示出51%的同一性。已知在FNR蛋白中至关重要的四个半胱氨酸残基在ANR中是保守的。通过最大细胞法观察到anr基因产物（推导的Mr为27,129），并且在变性条件下的凝胶电泳中迁移时表现得像一种32 kDa的蛋白质。通过基因替换构建的铜绿假单胞菌anr突变体在硝酸盐呼吸、精氨酸脱亚氨酶活性和氰化氢生物合成方面存在缺陷，这突出了ANR在氧限制期间的多种代谢功能。荧光假单胞菌、恶臭假单胞菌、丁香假单胞菌和门多萨假单胞菌都有ANR的功能类似物，表明这些细菌中存在类似的厌氧控制机制。
