Gorfinkiel Nicole, Arias Alfonso Martinez
Department of Genetics, University of Cambridge, Cambridge, CB2 3EH, UK.
J Cell Sci. 2007 Sep 15;120(Pt 18):3289-98. doi: 10.1242/jcs.010850.
Dynamic interactions between epithelial sheets are a regular feature of morphogenetic processes. Dorsal closure in Drosophila relies on the coordinated movements of two epithelia, the epidermis and the amnioserosa, and provides an excellent model system for a genetic and cell biological approach. Here, we have analyzed the contribution of junctional organization of these epithelia to dorsal closure. We observe a stringent requirement for adherens junctions at the leading edge, the interface between the amnioserosa and the epidermis, for the transmission of the forces generated during the process. We also find that interactions between Armadillo and E-cadherin play an important role in maintaining the adhesion at the leading edge, revealing the particular dynamics of this interface. Our results show that regulated cell adhesion is a crucial element of the interactions that shape epithelial sheets in morphogenetic processes.
上皮细胞层之间的动态相互作用是形态发生过程的一个常见特征。果蝇的背侧闭合依赖于两种上皮细胞（表皮和羊膜浆膜）的协调运动，为遗传和细胞生物学方法提供了一个出色的模型系统。在这里，我们分析了这些上皮细胞的连接组织对背侧闭合的贡献。我们观察到，在前缘（羊膜浆膜和表皮之间的界面），对于该过程中产生的力的传递，紧密连接有严格的要求。我们还发现，犰狳蛋白和E-钙黏蛋白之间的相互作用在维持前缘的黏附中起重要作用，揭示了该界面的特殊动态。我们的结果表明，受调控的细胞黏附是在形态发生过程中塑造上皮细胞层的相互作用的关键要素。
