Blüher Matthias, Williams Catherine J, Klöting Nora, Hsi Alex, Ruschke Karen, Oberbach Andreas, Fasshauer Mathias, Berndt Janin, Schön Michael R, Wolk Alicja, Stumvoll Michael, Mantzoros Christos S
Department of Internal Medicine III, University of Leipzig, Leipzig, Germany.
Diabetes Care. 2007 Dec;30(12):3110-5. doi: 10.2337/dc07-1257. Epub 2007 Sep 18.
Adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2, respectively) mediate the effects of adiponectin on glucose and lipid metabolism in vivo. We examined whether AdipoR1 and/or AdipoR2 mRNA expression in human adipose tissue is fat-depot specific. We also studied whether their expression in visceral and subcutaneous fat depots is associated with metabolic parameters and whether their expression is regulated by intensive physical exercise.
We determined metabolic parameters and assessed AdipoR1 and AdipoR2 mRNA expression using quantitative real-time PCR in adipose tissue in an observational study of 153 subjects and an interventional study of 60 subjects (20 each with normal glucose tolerance, impaired glucose tolerance, and type 2 diabetes) before and after intensive physical training for 4 weeks.
AdipoR1 and AdipoR2 mRNA expression is not significantly different between omental and subcutaneous fat, but their expression is several-fold lower in adipose tissue than in muscle. AdipoR2 mRNA expression in visceral fat is highly correlated with its expression in subcutaneous fat. AdipoR2 mRNA expression in both visceral and subcutaneous fat is positively associated with circulating adiponectin and HDL levels but negatively associated with obesity as well as parameters of insulin resistance, glycemia, and other lipid levels before and after adjustment for fat mass. Physical training for 4 weeks resulted in increased AdipoR1 and AdipoR2 mRNA expression in subcutaneous fat.
AdipoR2 mRNA expression in fat is negatively associated with insulin resistance and metabolic parameters independently of obesity and may mediate the improvement of insulin resistance in response to exercise.
脂联素受体1和2（分别为AdipoR1和AdipoR2）介导脂联素在体内对葡萄糖和脂质代谢的作用。我们研究了人脂肪组织中AdipoR1和/或AdipoR2 mRNA表达是否具有脂肪储存特异性。我们还研究了它们在内脏和皮下脂肪储存中的表达是否与代谢参数相关，以及它们的表达是否受高强度体育锻炼的调节。
在一项对153名受试者的观察性研究和一项对60名受试者（分别有20名糖耐量正常、糖耐量受损和2型糖尿病患者）的干预性研究中，我们在4周高强度体育训练前后测定了代谢参数，并使用定量实时PCR评估了脂肪组织中AdipoR1和AdipoR2 mRNA的表达。
网膜脂肪和皮下脂肪中AdipoR1和AdipoR2 mRNA表达无显著差异，但它们在脂肪组织中的表达比在肌肉中低几倍。内脏脂肪中AdipoR2 mRNA表达与其在皮下脂肪中的表达高度相关。在内脏和皮下脂肪中，AdipoR2 mRNA表达与循环脂联素和高密度脂蛋白水平呈正相关，但在调整脂肪量前后，与肥胖以及胰岛素抵抗、血糖和其他脂质水平参数呈负相关。4周的体育训练导致皮下脂肪中AdipoR1和AdipoR2 mRNA表达增加。
脂肪中AdipoR2 mRNA表达与胰岛素抵抗和代谢参数呈负相关，独立于肥胖，可能介导运动引起的胰岛素抵抗改善。
