Ricci Camilla, Eliasson Charlotte, Macleod Neil A, Newton Paul N, Matousek Pavel, Kazarian Sergei G
Department of Chemical Engineering, Imperial College London, London SW7 2AZ, UK.
Anal Bioanal Chem. 2007 Nov;389(5):1525-32. doi: 10.1007/s00216-007-1543-1. Epub 2007 Sep 19.
In support of the efforts to combat the illegal sale and distribution of counterfeit anti-malarial drugs, we evaluated a new analytical approach for the characterization and fast screening of fake and genuine artesunate tablets using a combination of Raman spectroscopy, Spatially Offset Raman Spectroscopy (SORS) and Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) imaging. Vibrational spectroscopy provided chemically specific information on the composition of the tablets; the complementary nature of Raman scattering and FTIR imaging allowed the characterization of both the overall and surface composition of the tablets. The depth-resolving power of the SORS approach provided chemically specific information on the overall composition of the tablets, non-invasively, through a variety of packaging types. Spatial imaging of the tablet surface (using ATR-FTIR) identified the location of domains of excipients and active ingredients with high sensitivity and enhanced spatial resolution. The advantages provided by a combination of SORS and ATR-FTIR imaging in this context confirm its potential for inclusion in the analytical protocol for forensic investigation of counterfeit medicines.
为支持打击假冒抗疟药物非法销售和分销的工作，我们评估了一种新的分析方法，该方法结合拉曼光谱、空间偏移拉曼光谱（SORS）和衰减全反射傅里叶变换红外（ATR-FTIR）成像技术，用于鉴别真假青蒿琥酯片并进行快速筛选。振动光谱提供了片剂成分的化学特异性信息；拉曼散射和傅里叶变换红外成像的互补特性使得能够对片剂的整体成分和表面成分进行表征。SORS方法的深度分辨能力通过多种包装类型，以非侵入方式提供了片剂整体成分的化学特异性信息。片剂表面的空间成像（使用ATR-FTIR）以高灵敏度和增强的空间分辨率确定了辅料和活性成分区域的位置。在这种情况下，SORS和ATR-FTIR成像相结合所提供的优势证实了其被纳入假冒药品法医调查分析方案的潜力。
