Bex1基因敲除小鼠表现出骨骼肌再生改变。
Bex1 knock out mice show altered skeletal muscle regeneration.
作者信息
Koo Jae Hyung, Smiley Mark A, Lovering Richard M, Margolis Frank L
机构信息
Department of Anatomy and Neurobiology, School of Medicine, University of Maryland Baltimore, Baltimore, MD 21201, USA.
出版信息
Biochem Biophys Res Commun. 2007 Nov 16;363(2):405-10. doi: 10.1016/j.bbrc.2007.08.186. Epub 2007 Sep 11.
Bex1 and Calmodulin (CaM) are upregulated during skeletal muscle regeneration. We confirm this finding and demonstrate the novel finding that they interact in a calcium-dependent manner. To study the role of Bex1 and its interaction with CaM in skeletal muscle regeneration, we generated Bex1 knock out (Bex1-KO) mice. These mice appeared to develop normally and are fertile, but displayed a functional deficit in exercise performance compared to wild type (WT) mice. After intramuscular injection of cardiotoxin, which causes extensive and reproducible myotrauma followed by recovery, regenerating muscles of Bex1-KO mice exhibited elevated and prolonged cell proliferation, as well as delayed cell differentiation, compared to WT mice. Thus, our results provide the first evidence that Bex1-KO mice show altered muscle regeneration, and allow us to propose that the interaction of Bex1 with Ca(2+)/CaM may be involved in skeletal muscle regeneration.
在骨骼肌再生过程中，Bex1和钙调蛋白（CaM）表达上调。我们证实了这一发现，并展示了它们以钙依赖方式相互作用的新发现。为了研究Bex1的作用及其与CaM在骨骼肌再生中的相互作用，我们构建了Bex1基因敲除（Bex1-KO）小鼠。这些小鼠似乎发育正常且可育，但与野生型（WT）小鼠相比，其运动能力存在功能缺陷。在肌肉内注射引起广泛且可重复的肌肉损伤随后恢复的心脏毒素后，与WT小鼠相比，Bex1-KO小鼠再生肌肉的细胞增殖升高且持续时间延长，以及细胞分化延迟。因此，我们的结果提供了首个证据，即Bex1-KO小鼠表现出肌肉再生改变，并使我们提出Bex1与Ca(2+)/CaM的相互作用可能参与骨骼肌再生。
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