动脉高血压会加剧早期糖尿病视网膜病变中的氧化应激。

Arterial hypertension exacerbates oxidative stress in early diabetic retinopathy.

作者信息

Pinto Camila C, Silva Kamila C, Biswas Subrata K, Martins Natássia, De Faria José B Lopes, De Faria Jacqueline M Lopes

机构信息

Renal Pathophysiology Laboratory, Department of Internal Medicine, Faculty of Medical Sciences (FCM), State University of Campinas, Campinas, São Paulo, Brazil.

出版信息

Free Radic Res. 2007 Oct;41(10):1151-8. doi: 10.1080/10715760701632816.

DOI:10.1080/10715760701632816

PMID:17886037

Abstract

The present study was undertaken to investigate the redox status in the retina of an experimental model that combines hypertension and diabetes. Spontaneously hypertensive rats (SHR) and their control Wystar Kyoto (WKY) rats were rendered diabetic and, after 20 days, the rats were sacrificed and the retinas collected. The superoxide production was higher in diabetic than in control WKY (p < 0.03) and SHR rats showed elevated superoxide production compared with WKY groups (p < 0.009). The glutathione antioxidant system was diminished only in diabetic SHR (p < 0.04). Tirosyne nitration was higher in diabetic WKY and control SHR compared with control WKY (p < 0.03), and further increment was observed in diabetic SHR (p < 0.02). The DNA damage estimated by immunohystochemistry for 8-OHdG was higher in control SHR than in WKY, mainly in diabetic SHR (p < 0.0001). Hypertension aggravates oxidative-induced cytotoxicity in diabetic retina due to increasing of superoxide production and impairment of antioxidative system.

摘要

本研究旨在调查合并高血压和糖尿病的实验模型视网膜中的氧化还原状态。将自发性高血压大鼠（SHR）及其对照Wystar Kyoto（WKY）大鼠诱导成糖尿病模型，20天后，处死大鼠并收集视网膜。糖尿病大鼠的超氧化物生成量高于对照WKY大鼠（p < 0.03），与WKY组相比，SHR大鼠的超氧化物生成量升高（p < 0.009）。仅糖尿病SHR大鼠的谷胱甘肽抗氧化系统减少（p < 0.04）。与对照WKY大鼠相比，糖尿病WKY大鼠和对照SHR大鼠的酪氨酸硝化水平更高（p < 0.03），糖尿病SHR大鼠中观察到进一步升高（p < 0.02）。通过免疫组织化学检测8-OHdG估计的DNA损伤在对照SHR大鼠中高于WKY大鼠，主要是在糖尿病SHR大鼠中（p < 0.0001）。高血压由于超氧化物生成增加和抗氧化系统受损，加剧了糖尿病视网膜中氧化诱导的细胞毒性。