通过生物共轭量子点对间充质干细胞进行标记
Labeling of mesenchymal stem cells by bioconjugated quantum dots.
作者信息
Shah Bhranti S, Clark Paul A, Moioli Eduardo K, Stroscio Michael A, Mao Jeremy J
机构信息
College of Dental Medicine, Department of Biomedical Engineering, Fu Foundation School of Engineering and Applied Sciences, Columbia University, New York, New York 10032, USA.
出版信息
Nano Lett. 2007 Oct;7(10):3071-9. doi: 10.1021/nl071547f. Epub 2007 Sep 22.
Abstract
Long-term labeling of stem cells during self-replication and differentiation benefits investigations of development and tissue regeneration. We report the labeling of human mesenchymal stem cells (hMSCs) with RGD-conjugated quantum dots (QDs) during self-replication, and multilineage differentiations into osteogenic, chondrogenic, and adipogenic cells. QD-labeled hMSCs remained viable as unlabeled hMSCs from the same subpopulation. These findings suggest the use of bioconjugated QDs as an effective probe for long-term labeling of stem cells.
摘要
在自我复制和分化过程中对干细胞进行长期标记，有利于开展发育和组织再生研究。我们报告了在人骨髓间充质干细胞（hMSCs）自我复制期间，用RGD缀合量子点（QDs）对其进行标记，并使其多向分化为成骨细胞、软骨细胞和脂肪细胞。量子点标记的hMSCs与来自相同亚群的未标记hMSCs一样保持活力。这些发现表明，生物缀合量子点可作为干细胞长期标记的有效探针。
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