Lapan G, Awad-Masalmeh M, Hartig A, Silber R
Institut für Bakteriologie und Tierhygiene, Veterinärmedizinischen Universität Wien.
Zentralbl Veterinarmed B. 1991 Oct;38(8):589-98.
In this study 55 strains of Taylorella equigenitalis isolated from horses of four different studs in Austria, and a comparative strain from the Federal Republic of Germany were investigated by different methods. These investigations were carried out with the help of SDS-PAGE, immunoblotting, the analyses of genomes and by proof of plasmids. Furthermore, pathogenic mechanisms such as adhesion or the formation of toxins were investigated in vitro. On the basis of the results carried out by means of SDS-PAGE and immunoblotting all tested strains of Taylorella equigenitalis were alike, whereas by DNA analyses the strains could be divided into five groups. The comparative strain from the FRG, which clearly differed from the Austrian strains, formed one group all by itself. From three studs, which are related to each other because of an intensive exchange of horses, representatives (n = 53) of three DNA fingerprint groups were isolated. These three fingerprint patterns were very similar to each other, while the hybridisation patterns from the other two Austrian strains were very different. One of these strains, isolated from a diseased mare, could not be distinguished from the other strain isolated from a clinical healthy stallion from the same study by this method. Only 47.3% from the investigated strains showed attachment to HeLa cells, while cell extracts of all of them caused morphological changes of a varying degree of both Y1 and Vero cells. There were no connexions between these adhesion-cytotoxicity-properties and the DNA fingerprint groups as well as the studs, respectively. No plasmids were found in the Taylorella equigenitalis strains used in this study.
在本研究中，对从奥地利四个不同种马场的马匹中分离出的55株马生殖道泰勒菌，以及一株来自德意志联邦共和国的对照菌株，采用不同方法进行了研究。这些研究借助十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、免疫印迹、基因组分析和质粒检测来开展。此外，还在体外研究了诸如黏附或毒素形成等致病机制。基于SDS-PAGE和免疫印迹的结果，所有测试的马生殖道泰勒菌菌株均相似，而通过DNA分析，这些菌株可分为五组。来自德意志联邦共和国的对照菌株与奥地利菌株明显不同，自成一组。在因马匹频繁交换而相互关联的三个种马场中，分离出了三个DNA指纹图谱组的代表菌株（n = 53）。这三种指纹图谱彼此非常相似，而另外两株奥地利菌株的杂交图谱则差异很大。其中一株从患病母马分离出的菌株，用此方法无法与同一研究中从临床健康种公马分离出的另一菌株区分开来。在所研究的菌株中，只有47.3%的菌株显示出对HeLa细胞的黏附，而它们所有的细胞提取物均导致Y1和Vero细胞出现不同程度的形态变化。这些黏附-细胞毒性特性与DNA指纹图谱组以及种马场之间均无关联。在本研究使用的马生殖道泰勒菌菌株中未发现质粒。
