Matsuda M, Miyazawa T, Moore J E, Buckley T C, Thomas L A
Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
Vet Res Commun. 1998 Jun;22(4):217-24. doi: 10.1023/a:1006002212604.
The profiles, after digestion with ApaI or NotI and pulsed-field gel electrophoresis (PFGE), of genomic DNA from 18 strains of Taylorella equigenitalis isolated in Ireland were of three different types. The 13 strains in one of these types gave PFGE profiles identical to that of an American prototype strain, Kentucky 188, but different from strain NCTC11184T and from a strain isolated in Japan. Eight additional strains isolated in the United States gave four distinct types of genomic PFGE profiles. All four types were different from that of T. equigenitalis NCTC11184T or that of the Japanese strain. The profile of three strains of one type was identical to that of Kentucky 188.
用ApaI或NotI消化并经脉冲场凝胶电泳（PFGE）分析后，从爱尔兰分离出的18株马生殖道泰勒菌的基因组DNA图谱分为三种不同类型。其中一种类型的13株菌株产生的PFGE图谱与美国原型菌株肯塔基188相同，但与菌株NCTC11184T以及从日本分离出的一株菌株不同。在美国分离出的另外8株菌株产生了四种不同类型的基因组PFGE图谱。所有这四种类型均与马生殖道泰勒菌NCTC11184T或日本菌株的图谱不同。其中一种类型的三株菌株的图谱与肯塔基188相同。
