Matsuda M, Miyazawa T, Ishida Y, Moore J E
Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
Microbiol Res. 1997 Jul;152(2):217-20. doi: 10.1016/S0944-5013(97)80015-8.
The genomic DNA of eight strains of Taylorella equigenitalis, isolated from seven Norwegian Trotters and a Norwegian pony with contagious equine metritis in Norway, was examined by pulsed-field gel electrophoresis after separate digestions with two restriction enzymes, namely, ApaI and NotI. The respective electrophoretic profiles of the fragments were essentially identical but differed from those of T. equigenitalis NCTC11184T and Kentucky 188. They also exhibited slight differences from profiles obtained from Japanese isolates. These results may possibly suggest a common genotype and a common source of infection for all these eight isolates in Norway.
从挪威的7匹挪威快步马和1匹患有传染性马子宫内膜炎的挪威矮种马中分离出8株马生殖器泰勒菌，其基因组DNA在用两种限制性内切酶（即ApaI和NotI）分别消化后，通过脉冲场凝胶电泳进行检测。片段的各自电泳图谱基本相同，但与马生殖器泰勒菌NCTC11184T和肯塔基188的图谱不同。它们与从日本分离株获得的图谱也有细微差异。这些结果可能表明挪威的所有这8株分离株具有共同的基因型和共同的感染源。
