Cabral G A, Schaffer P A
J Virol. 1976 May;18(2):727-37. doi: 10.1128/JVI.18.2.727-737.1976.
Nine temperature-sensitive mutants of herpes simplex virus type 2 representing eight complementation groups were assigned to two classes as a consequence of the virion forms and virus-specific cellular alterations observed in thin sections of mutant-infected human embryonic lung cells grown at the nonpermissive temperature. Mutants in class A, one DNA- and one DNA +, failed to synthesize detectable virus particles. Mutants in class B, 4DNA- and 3DNA+, produced moderate to large numbers of empty nucleocapsids. Dense-cored nucleocapsids were not observed in thin sections of cells infected with any of the nine mutants at this temperature. Virus-specific cellular alterations consisted primarily of margination of chromating and nulcear membrane thickening and duplication.
由于在非允许温度下生长的突变体感染的人胚肺细胞超薄切片中观察到的病毒体形式和病毒特异性细胞改变，代表八个互补群的九个2型单纯疱疹病毒温度敏感突变体被分为两类。A类突变体，一个DNA -和一个DNA +，未能合成可检测到的病毒颗粒。B类突变体，4个DNA -和3个DNA +，产生中等数量到大量的空核衣壳。在此温度下，用这九个突变体中的任何一个感染的细胞超薄切片中均未观察到致密核心核衣壳。病毒特异性细胞改变主要包括染色质边缘化、核膜增厚和复制。
