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[用于黄酮类化合物薄层色谱分离的表面活性剂研究]

[Studies on surfactants used for thin layer chromatographic separation of flavones].

作者信息

Lin H G, Ge Z C, Li Z L, Yu R Q

机构信息

Department of Chemistry, Hunan University, Changsha.

出版信息

Yao Xue Xue Bao. 1991;26(6):471-4.

PMID:1789116
Abstract

The micellar thin layer chromatographic behaviours of rutin, quercetin and morin were studied. The methods of separation and identification were established: Polyamide thin coating was used as the stationary phase and 4% Zeph--acetylacetone--H2O (5:1.5:1.5) used as the mobile phase. The method was applied to the separation and identification of quercetin and rutin in samples of Sophorae flos and satisfactory result was obtained.

摘要

研究了芦丁、槲皮素和桑色素的胶束薄层色谱行为。建立了分离和鉴定方法:以聚酰胺薄涂层为固定相,4%溴化十六烷基三甲铵 - 乙酰丙酮 - 水(5:1.5:1.5)为流动相。该方法应用于槐花样品中槲皮素和芦丁的分离与鉴定,获得了满意的结果。

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