DeMarchi J M, Kaplan A S
J Virol. 1976 Jun;18(3):1063-70. doi: 10.1128/JVI.18.3.1063-1070.1976.
Human cytomegalovirus (CMV) DNA synthesis was studied in 5-fluorouracil (FU)-treated and untreated human embryonic lung cells, which differ greatly with respect to the number of cells in the culture synthesizing cellular DNA. CMV DNA synthesis proceeded at the same rate in FU-treated and in untreated cells. CMV infection also reversed the inhibitory effects of FU and activated cellular DNA synthesis in some of the cells in the FU-treated culture. Autoradiographic studies showed that more than 20% of the cells in the infected FU-treated culture synthesized viral DNA when less than 1% had synthesized cellular DNA, indicating that the synthesis of viral macromolecules proceeds in cells that do not synthesize cellular DNA from the time of infection, and that viral DNA synthesis proceeds independently of the host cell DNA synthesis. Combined autoradiographic and immunofluorescence studies of both the FU-treated and untreated infected cells showed that, whereas 20% of the cells in the cultures synthesize viral DNA and viral antigens, only about 3 to 6% of those cells that synthesize cellular DNA also synthesize viral antigen. Thus, productive infection was delayed or inhibited in those cells that were stimulated by CMV infection to synthesize cellular DNA.
在经5-氟尿嘧啶（FU）处理和未处理的人胚肺细胞中研究了人巨细胞病毒（CMV）的DNA合成，这两种细胞在合成细胞DNA的培养细胞数量方面差异很大。CMV DNA合成在经FU处理的细胞和未处理的细胞中以相同的速率进行。CMV感染还逆转了FU的抑制作用，并激活了经FU处理的培养物中一些细胞的细胞DNA合成。放射自显影研究表明，在受感染的经FU处理的培养物中，当合成细胞DNA的细胞少于1%时，超过20%的细胞合成了病毒DNA，这表明从感染时起，病毒大分子的合成就在不合成细胞DNA的细胞中进行，并且病毒DNA合成独立于宿主细胞DNA合成进行。对经FU处理和未处理的受感染细胞进行的放射自显影和免疫荧光联合研究表明，虽然培养物中20%的细胞合成病毒DNA和病毒抗原，但在那些合成细胞DNA的细胞中，只有约3%至6%的细胞也合成病毒抗原。因此，在被CMV感染刺激而合成细胞DNA的那些细胞中，生产性感染被延迟或抑制。
