Kaiserman Igor, Bahar Irit, Rootman David S
Department of Ophthalmology, Toronto Western Hospital, University of Toronto, Toronto, Ontario, Canada.
Cornea. 2007 Oct;26(9):1115-7. doi: 10.1097/ICO.0b013e318142bdb9.
To image Descemet membrane separation by the big bubble technique in human corneas by using anterior segment optical coherence tomography (OCT).
Five human corneoscleral rims were placed on an artificial anterior chamber and partially trephinated. A 23-gauge needle was inserted into the stroma under slit-lamp control and air was injected. The procedure was continuously imaged by anterior segment OCT.
In all corneoscleral rims, a big bubble was created. The spread of air seemed to follow the interlamellar spaces without crossing lamellae. It involved mainly the inner layers of the stroma while sparing the outer 212 +/- 41 microm of the cornea (range, 168-271 microm). Intrastromal pressure build-up forced air above the Descemet membrane, creating tiny air bubbles of approximately 355 +/- 111 microm (range, 210-560 microm). When the pressure inside those bubbles reached a certain level, the bubbles spontaneously coalesced to form a big bubble.
OCT is useful in imaging intracorneal air spread. The main obstacle to creating a big bubble is the impermeability to air of the imperforated posterior stromal lamellae.
通过眼前节光学相干断层扫描（OCT）对人眼角膜采用大泡技术进行后弹力层分离成像。
将5个人类角膜缘组织置于人工前房并部分环钻。在裂隙灯控制下将23号针头插入基质层并注入空气。该过程通过眼前节OCT进行连续成像。
在所有角膜缘组织中均形成了一个大泡。空气的扩散似乎沿着板层间隙进行，未穿过板层。它主要累及基质层的内层，而角膜外层212±41微米（范围168 - 271微米）未受影响。基质内压力升高迫使空气进入后弹力层上方，形成约355±111微米（范围210 - 560微米）的微小气泡。当这些气泡内的压力达到一定水平时，气泡会自发合并形成一个大泡。
OCT有助于对角膜内空气扩散进行成像。形成大泡的主要障碍是未穿孔的后基质板层对空气的不渗透性。
