Krwawicz Joanna, Arczewska Katarzyna D, Speina Elzbieta, Maciejewska Agnieszka, Grzesiuk Elzbieta
Department of Molecular Biology, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warszawa, Poland.
Acta Biochim Pol. 2007;54(3):413-34. Epub 2007 Sep 24.
Base excision repair (BER) pathway executed by a complex network of proteins is the major system responsible for the removal of damaged DNA bases and repair of DNA single strand breaks (SSBs) generated by environmental agents, such as certain cancer therapies, or arising spontaneously during cellular metabolism. Both modified DNA bases and SSBs with ends other than 3'-OH and 5'-P are repaired either by replacement of a single or of more nucleotides in the processes called short-patch BER (SP-BER) or long-patch BER (LP-BER), respectively. In contrast to Escherichia coli cells, in human ones, the two BER sub-pathways are operated by different sets of proteins. In this review the selection between SP- and LP-BER and mutations in BER and end-processors genes and their contribution to bacterial mutagenesis and human diseases are considered.
由复杂的蛋白质网络执行的碱基切除修复（BER）途径是负责去除受损DNA碱基以及修复由环境因素（如某些癌症治疗手段）或细胞代谢过程中自发产生的DNA单链断裂（SSB）的主要系统。修饰的DNA碱基和末端不是3'-OH和5'-P的SSB，分别通过在称为短补丁BER（SP-BER）或长补丁BER（LP-BER）的过程中替换单个或多个核苷酸来修复。与大肠杆菌细胞不同，在人类细胞中，这两个BER子途径由不同的蛋白质组来运作。在这篇综述中，我们考虑了SP-BER和LP-BER之间的选择、BER和末端加工基因中的突变及其对细菌诱变和人类疾病的影响。
