Read Randy J, Chavali Gayatri
Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
Proteins. 2007;69 Suppl 8:27-37. doi: 10.1002/prot.21662.
Models for target domains in the high accuracy template-based modeling category were assessed according to a number of criteria evaluating the quality of the main-chain prediction (GDT-HA), predicted sequence alignment (AL0), and side-chain rotameric state. A new criterion was introduced, the quality of the model for use in solving a crystal structure by molecular replacement. There is good evidence that modeling adds value to the template structures, particularly when multiple templates are available. However, when there is already a good template, few of the models are better for the purpose of molecular replacement.
基于高精度模板的建模类别中目标域的模型，根据评估主链预测质量（GDT-HA）、预测序列比对（AL0）和侧链旋转异构体状态的若干标准进行评估。引入了一个新的标准，即用于通过分子置换解析晶体结构的模型质量。有充分证据表明，建模为模板结构增加了价值，尤其是当有多个模板可用时。然而，当已经有一个好的模板时，很少有模型在分子置换方面表现得更好。
