Laderas Ted, Bystrom Cory, McMillen Debra, Fan Guang, McWeeney Shannon
Informatics Shared Resource, OHSU Cancer Institute, Oregon Health & Science University, Portland, Oregon 97212, USA.
Bioinformatics. 2007 Dec 15;23(24):3394-6. doi: 10.1093/bioinformatics/btm467. Epub 2007 Sep 25.
Integrating qualitative protein identification with quantitative protein analysis is non-trivial, given incompatibility in output formats. We present TandTRAQ, a standalone utility that integrates results from i-Tracker, an open-source iTRAQ quantitation program with the search results from X?Tandem, an open-source proteome search engine. The utility runs from the command-line and can be easily integrated into a pipeline for automation.
The TandTRAQ Perl scripts are freely available for download at http://www.ohsucancer.com/isrdev/tandtraq/
鉴于输出格式不兼容,将定性蛋白质鉴定与定量蛋白质分析相结合并非易事。我们展示了TandTRAQ,这是一个独立实用程序,它将来自开源iTRAQ定量程序i-Tracker的结果与来自开源蛋白质组搜索引擎X!Tandem的搜索结果整合在一起。该实用程序通过命令行运行,并且可以轻松集成到管道中以实现自动化。
TandTRAQ Perl脚本可从http://www.ohsucancer.com/isrdev/tandtraq/免费下载。