Balasubramanian Ashwin K, Soni Kamlesh A, Beskok Ali, Pillai Suresh D
Old Dominion University Aerospace Engineering Department, Norfolk, VA 23529-0247, USA.
Lab Chip. 2007 Oct;7(10):1315-21. doi: 10.1039/b706559k. Epub 2007 Jul 20.
A microfluidic device based on electrophoretic transport and electrostatic trapping of charged particles has been developed for continuous capture and concentration of microorganisms from water. Reclaimed and bottled water samples at pH values ranging from 5.2-6.5 were seeded with bacteria (E. coli, Salmonella, and Pseudomonas) and viruses (MS-2 and Echovirus). Negative control and capture experiments were performed simultaneously using two identical devices. Culture based methods were utilized to characterize the capture efficiency as a function of the species type, time, flow rate, and applied electric field. Based on differences between the capture and negative control data, capture efficiencies of 90% to 99% are reported for E. coli, Salmonella, Pseudomonas, and MS-2, while the capture efficiency for Echovirus was between 70% and 80%. Overall, the device exhibits a 16.67 fold sample volume reduction within an hour at 6 mL h(-1) flow rate, resulting in a concentration factor of 14.2 at 85.2% capture efficiency. The device can function either as a filter or a sample concentrator without using any chemical additives. It can function as an integral component of a continuous, microbial capture and concentration system from large volumes of potable water.
一种基于带电粒子电泳传输和静电捕获的微流控装置已被开发出来，用于从水中连续捕获和浓缩微生物。在pH值为5.2 - 6.5的再生水和瓶装水样品中接种细菌（大肠杆菌、沙门氏菌和假单胞菌）和病毒（MS - 2和埃可病毒）。使用两个相同的装置同时进行阴性对照和捕获实验。采用基于培养的方法来表征捕获效率与物种类型、时间、流速和外加电场的函数关系。根据捕获数据和阴性对照数据之间的差异，报告大肠杆菌、沙门氏菌、假单胞菌和MS - 2的捕获效率为90%至99%，而埃可病毒的捕获效率在70%至80%之间。总体而言，该装置在流速为6 mL h(-1)时，一小时内样品体积减少了16.67倍，在捕获效率为85.2%时浓缩系数为14.2。该装置无需使用任何化学添加剂即可作为过滤器或样品浓缩器发挥作用。它可作为从大量饮用水中连续捕获和浓缩微生物系统的一个组成部分。
