Puri S C, Amna T, Khajuria A, Gupta A, Arora Rajesh, Spiteller M, Qazi G N
Division of Natural Products Chemistry, Regional Research Laboratory (CSIR), Canal Road, Jammu-180001, India.
Acta Microbiol Immunol Hung. 2007 Sep;54(3):237-60. doi: 10.1556/AMicr.54.2007.3.2.
A novel camptothecin-producing endophytic fungus viz., Entrophospora infrequens was isolated from an important Indian medicinal plant Nothapodytes foetida. The present study reports evaluation ofbioactivities of two novel extracts viz., chloroform (CEEI) and methanolic (MEEI) extracts of Entrophospora infrequens with respect to their immunomodulatory potential in vitro and in vivo (in Balb/c mice). The endophyte E. infrequens was found to synthesize camptothecin, which tested positive in CEEI. The immunomodulatory potential of CEEI and MEEI was compared with standard camptothecin (CPT). Doses of the chloroform extract (CEEI) ranging from 12.5-100 mg/kg body weight, significantly (p < 0.05) stimulated the humoral and cell-mediated immune responses in a dose-dependent manner. MEEI on the other hand significantly (p < 0.05) stimulated the delayed type hypersensitivity (DTH) reaction (by nearly 80%), plaque forming cell (PFC) assay (33%), phagocytic response (38%) and haemagglutination antibody (HA) titre [IgM by 79.07% and IgG by 62.05%] at a dose of 12.5 mg/kg body weight. The present study is the first report of the immunomodulatory potential of this neoteric camptothecin-producing endophyte from Nothapodytes foetida.
一种新型产喜树碱的内生真菌，即罕见内脐蠕孢菌，是从印度一种重要的药用植物臭假柴龙树中分离得到的。本研究报告了两种新型提取物，即罕见内脐蠕孢菌的氯仿提取物（CEEI）和甲醇提取物（MEEI）在体外和体内（在Balb/c小鼠中）免疫调节潜力的生物活性评估。发现内生菌罕见内脐蠕孢菌能合成喜树碱，其在CEEI中检测呈阳性。将CEEI和MEEI的免疫调节潜力与标准喜树碱（CPT）进行了比较。氯仿提取物（CEEI）剂量范围为12.5 - 100 mg/kg体重，以剂量依赖性方式显著（p < 0.05）刺激体液免疫和细胞介导的免疫反应。另一方面，MEEI在剂量为12.5 mg/kg体重时，显著（p < 0.05）刺激迟发型超敏反应（DTH）（提高近80%）、空斑形成细胞（PFC）测定（提高33%）、吞噬反应（提高38%）和血凝抗体（HA）滴度[IgM提高79.07%，IgG提高62.05%]。本研究是关于这种来自臭假柴龙树的新型产喜树碱内生菌免疫调节潜力的首次报道。
