Chen Tong, Zeng Shui-qing, Lu Ying-yi, Huang Lin-ying, Dai Hong
Department of Ophthalmology, Union Hospital, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430022, China.
Zhonghua Yan Ke Za Zhi. 2007 Jul;43(7):622-5.
To compare the change of the level of the vascular endothelial growth factor (VEGF) in aqueous humor of patients with neovascular glaucoma (NVG) before and after anterior retinal cryotherapy and investigate the effects of the fluctuation of VEGF on the neovascularization of iris.
28 patients with neovascular glaucoma were undergone iris fluorescent angiography to identify the area and amount of new vessels before and after anterior retinal cryotherapy. The neovascularization of iris was observed from 7 to 14 days by iris fluorescent angiography to confirm the regression of new vessels in iris before trabeculectomy. Samples of aqueous humor were obtained before anterior retinal cryotherapy and trabeculectomy, and 30 samples of aqueous humor from patients with senile cataract were collected as normal group. The concentrations of VEGF were measured using enzyme linked immunosorbent assay.
the concentration of VEGF (2.096 +/- 0.512) ng/ml in aqueous humor from patients with NVG were much higher than the specimen from the patients pre-trabeculectomy (0.478 +/- 0.312) ng/ml, There was a significant difference between the two groups (t = 17.994, P < 0.01). The mean VEGF concentration of the aqueous humor from patients with senile cataract was (0.198 +/- 0.045) ng/ml which was much lower compared with the samples from patients of pre-trabeculectomy (t = 18.453, P < 0.01).
The concentration of VEGF decline after the regression of new vessels in iris. The results suggest that VEGF play an important role in formation of iris neovascularization. Blockage the release of VEGF might reduce the occurrence of neovascular glaucoma.
比较新生血管性青光眼（NVG）患者视网膜冷凝术前、后房水中血管内皮生长因子（VEGF）水平的变化，探讨VEGF波动对虹膜新生血管形成的影响。
对28例新生血管性青光眼患者行虹膜荧光血管造影，观察视网膜冷凝术前、后虹膜新生血管的范围及数量。于视网膜冷凝术后7至14天通过虹膜荧光血管造影观察虹膜新生血管消退情况，以确定小梁切除术前行虹膜新生血管的消退情况。分别于视网膜冷凝术前及小梁切除术前行房水穿刺取样，同时收集30例老年性白内障患者的房水作为正常对照组。采用酶联免疫吸附测定法检测房水中VEGF浓度。
NVG患者房水中VEGF浓度为（2.096±0.512）ng/ml，明显高于小梁切除术前行房水标本中的（0.478±0.312）ng/ml，两组比较差异有统计学意义（t = 17.994，P < 0.01）。老年性白内障患者房水中VEGF平均浓度为（0.198±0.045）ng/ml，明显低于小梁切除术前行房水标本中的浓度（t = 18.453，P < 0.01）。
虹膜新生血管消退后房水中VEGF浓度下降。结果提示VEGF在虹膜新生血管形成中起重要作用。阻断VEGF释放可能减少新生血管性青光眼的发生。
