He Guimei, Qiao Jian, Dong Changgui, He Cheng, Zhao Lihong, Tian Yong
Department of Pathophysiology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, People's Republic of China.
Antiviral Res. 2008 Jan;77(1):72-6. doi: 10.1016/j.antiviral.2007.08.007. Epub 2007 Sep 12.
We tested the amantadine-resistance among avian influenza A (H5N1) viruses isolated from chicken in Hebei Province of Northern China from 2001 to 2005, and investigated the amantadine use in this area. Plague reduction assay in MDCK cells showed that 83.3% isolates (5/6) were amantadine-resistant strains. The M2 sequence analysis revealed that four of five resistant isolates contained the point mutations (Ser to Asn) at position 31 that could confer resistance to amantadine. These results indicated that the incidence of amantadine-resistant viruses isolated in Northern China was particularly high. In the investigation of amantadine use, we found that amantadine was used extensively in poultry farms in this area, which maybe was one of reasons of the high amantadine-resistance incidence.
我们检测了2001年至2005年从中国北方河北省鸡群中分离出的甲型禽流感（H5N1）病毒的金刚烷胺耐药性，并调查了该地区金刚烷胺的使用情况。在MDCK细胞中进行的蚀斑减少试验表明，83.3%的分离株（5/6）为金刚烷胺耐药株。M2序列分析显示，5株耐药分离株中有4株在第31位含有可导致对金刚烷胺耐药的点突变（丝氨酸突变为天冬酰胺）。这些结果表明，在中国北方分离出的金刚烷胺耐药病毒的发生率特别高。在对金刚烷胺使用情况的调查中，我们发现该地区的家禽养殖场广泛使用金刚烷胺，这可能是金刚烷胺耐药发生率高的原因之一。
