Drouet E B, Boude M, Denoyel G A
Department of Microbiology-Virology, Institut Pasteur de Lyon, France.
Zentralbl Bakteriol. 1991 Dec;276(1):9-15. doi: 10.1016/s0934-8840(11)80213-6.
The analysis of electrophoretic protein profiles of 21 Mobiluncus curtisii, 11 Mobiluncus mulieris and 3 reference strains (Mobiluncus curtisii subsp. curtisii ATCC 35241, Mobiluncus curtisii subsp. holmesii ATCC 35242 and Mobiluncus mulieris BV 64-5) demonstrated species-related patterns. A highly variable region appeared at 70-85 kDa for Mobiluncus curtisii and at 75-95 kDa for Mobiluncus mulieris, which was likely to correspond to cell surface located proteins. When performed under standardized conditions, PAGE-protein analysis allowed to define intraspecies clusters, from which some strains appeared identical. Thus, the method seemed to provide a useful additive to identify a strain at the species level and might be of epidemiological interest.
对21株柯氏动弯杆菌、11株穆氏动弯杆菌和3株参考菌株（柯氏动弯杆菌柯氏亚种ATCC 35241、柯氏动弯杆菌霍氏亚种ATCC 35242和穆氏动弯杆菌BV 64-5）的蛋白质电泳图谱分析显示出与菌种相关的模式。柯氏动弯杆菌在70 - 85 kDa处出现一个高度可变区域，穆氏动弯杆菌在75 - 95 kDa处出现一个高度可变区域，这可能对应于位于细胞表面的蛋白质。在标准化条件下进行时，PAGE - 蛋白质分析能够定义种内聚类，从中一些菌株看起来是相同的。因此，该方法似乎为在种水平上鉴定菌株提供了一种有用的辅助手段，并且可能具有流行病学意义。
