Skarin A
Acta Pathol Microbiol Immunol Scand B. 1986 Jun;94(3):127-33. doi: 10.1111/j.1699-0463.1986.tb03031.x.
Twenty-four strains of Mobiluncus mulieris and 27 strains of Mobiluncus curtisii were tested with respect to 6 different biochemical characteristics: arginin-decarboxylase activity, beta-galactosidase activity, synergistic hemolysis with Staphylococcus aureus, hydrolysis of hippurate, migration through soft agar and reduction of nitrate. Antigens of the same strains, prepared by ultrasonication, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting using polyclonal rabbit antisera against two of the M. mulieris strains and five of the M. curtisii strains. Two different strongly reacting protein antigens could be detected in the M. mulieris strains. These strains could be separated into three groups based on the possession of either of the two antigens or both. In the M. curtisii strains, 10 strongly reacting protein antigens could be detected. Four strains possessed only one of these antigens, one did not possess any, while the remaining strains possessed different sets of 9 of them. Within each species common protein antigens were detected. No antigens were found which were shared by both species. The biochemical characteristics studied could not differentiate between the antigenic groups in any of the species. None of the antigenic subgroups of M. curtisii found in the present study was identical with any of the two subspecies (curtisii and holmesii) which have been proposed.
针对24株穆氏动弯杆菌和27株柯氏动弯杆菌，检测了6种不同的生化特性：精氨酸脱羧酶活性、β-半乳糖苷酶活性、与金黄色葡萄球菌的协同溶血作用、马尿酸盐水解、在软琼脂中的迁移以及硝酸盐还原。通过超声处理制备相同菌株的抗原，然后进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，接着使用针对两株穆氏动弯杆菌菌株和五株柯氏动弯杆菌菌株的兔多克隆抗血清进行免疫印迹分析。在穆氏动弯杆菌菌株中可检测到两种不同的强反应性蛋白质抗原。根据拥有其中一种抗原、两种抗原或两者都有，这些菌株可分为三组。在柯氏动弯杆菌菌株中，可检测到10种强反应性蛋白质抗原。四株菌株仅拥有其中一种抗原，一株没有任何抗原，而其余菌株拥有其中9种不同组合的抗原。在每个菌种内都检测到了共同的蛋白质抗原。未发现两种菌种共有的抗原。所研究的生化特性无法区分任何一个菌种中的抗原组。在本研究中发现的柯氏动弯杆菌的任何一个抗原亚组都与已提出的两个亚种（柯氏亚种和霍氏亚种）中的任何一个不同。
