Biotechnology Centre, Indian Agricultural Research Institute, 110012, New Delhi, India.
Plant Cell Rep. 1994 Aug;13(11):657-60. doi: 10.1007/BF00232940.
Camelina sativa is a wild crucifer that is reported to be resistant to Alternaria blight. Polyethylene glycol mediated fusion was attempted between protoplasts from etiolated hypocotyls of Brassica carinata and mesophyll protoplasts of Camelina sativa. The mean frequency of heterokaryons was 6.8%. Three hybrid shoots were regenerated, each from a single fusionderived callus. These shoots failed to produce roots capable of withstanding transplantation. Confirmation of hybridity was obtained from the morphology of in vitro produced leaves, somatic chromosome number in leaf tips, and restriction fragment length polymorphism for a nuclear rDNA probe. Analysis for organelle constitution using RFLPs indicated that the hybrid contained chrloroplasts derived from the wild species and mitochondria from the cultivated Brassica species.
荠蓝是一种野生十字花科植物，据报道对交链孢黑斑病有抗性。尝试用聚乙二醇介导法将油菜黄化下胚轴原生质体和荠蓝叶肉原生质体融合。杂种核的平均频率为 6.8%。从单个融合衍生的愈伤组织中再生了 3 个杂种芽。这些芽未能产生能够耐受移植的根。杂种的确认是从体外产生的叶片的形态、叶尖体细胞染色体数和核 rDNA 探针的限制片段长度多态性获得的。使用 RFLP 对细胞器组成进行分析表明，杂种含有来自野生种的叶绿体和来自栽培的芸薹属种的线粒体。
