Chakravarti Anita, Matlani Monika, Jain Manisha
Department of Microbiology, Maulana Azad Medical College & Associated Lok Nayak Hospitals, 79, South Park Apartment, Kalkaji, New Delhi 110002, India.
Curr Microbiol. 2007 Dec;55(6):461-4. doi: 10.1007/s00284-007-9040-5. Epub 2007 Sep 27.
Keeping in view the complications and the case fatality associated with dengue virus, several serologic tests have been developed. However, the major drawback of these serologic tests is the need for a venous blood sample obtained by invasive venipuncture. As a noninvasive alternative, saliva provides a body fluid that contains antibodies of diagnostic importance. Hence, the detection of DEN-specific IgM and IgG antibodies in serum and saliva from 80 patients was compared. Salivary IgM antibodies were detected in 100% of the serum IgM-positive samples and in 30% of the serum samples that were negative for IgM antibodies. Salivary IgG antibodies were detected in 93.3% of the serum samples that were positive for anti-dengue IgG antibodies and in none of the serum IgG-negative cases. None of the specimens from the healthy controls showed the presence of IgM or IgG antibodies. The detection of both IgG and IgM antibodies in saliva correlated well with the serum IgG and IgM detection by the ELISA test (r = 0.6322 and r = 0.4227). Detection of salivary IgM antibodies by ELISA showed 100% sensitivity, 70% specificity, 90.9% positive predictive value, and 100% negative predictive value. The detection of IgG in saliva proved to be a promising tool as the sensitivity, specificity, positive predictive value, and negative predictive value were found out to be 93.3%, 100%, 100%, and 83.3%, respectively. Thus, from this study we conclude that the detection of DEN-specific salivary IgG and IgM antibodies are useful markers for dengue infection.
鉴于登革病毒相关的并发症和病死率，已开发了多种血清学检测方法。然而，这些血清学检测的主要缺点是需要通过侵入性静脉穿刺获取静脉血样本。作为一种非侵入性替代方法，唾液提供了一种含有具有诊断重要性抗体的体液。因此，对80例患者血清和唾液中登革病毒特异性IgM和IgG抗体的检测进行了比较。在100%的血清IgM阳性样本以及30%的血清IgM抗体阴性样本中检测到唾液IgM抗体。在93.3%的抗登革病毒IgG抗体阳性血清样本中检测到唾液IgG抗体，而在所有血清IgG阴性病例中均未检测到。健康对照的所有标本均未显示存在IgM或IgG抗体。唾液中IgG和IgM抗体的检测与ELISA检测血清IgG和IgM的结果相关性良好（r = 0.6322和r = 0.4227）。ELISA检测唾液IgM抗体的敏感性为100%，特异性为70%，阳性预测值为90.9%，阴性预测值为100%。唾液中IgG的检测被证明是一种有前景的工具，因为其敏感性、特异性、阳性预测值和阴性预测值分别为93.3%、100%、100%和83.3%。因此，从本研究中我们得出结论，检测登革病毒特异性唾液IgG和IgM抗体是登革热感染的有用标志物。
