State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2022 Jun 4;14(11):2347. doi: 10.3390/nu14112347.
Eighty-eight strains, which were isolated from human, chicken and cow fecal samples from different niches of China, were compared genomically in this study to evaluate their diversity. It was found that displayed a closed pan-genome, including abundant glycoside hydrolase families of the carbohydrate active enzyme (CAZy). A total of 30 kinds of glycoside hydrolases (GHs), 14 kinds of glycosyl transferases (GTs), 13 kinds of carbohydrate-binding modules (CBMs), 6 kinds of carbohydrate-esterases (CEs), and 2 kinds of auxiliary activities (AAs) gene families were identified across the genomes of the 88 strains. Specifically, this showed that significant differences were also present in the number of 10 carbohydrate-active enzyme gene families (GT51, GH13_32, GH26, GH42, GH121, GH3, AA3, CBM46, CE2, and CE6) among the strains derived from the hosts of different age groups, particularly between strains from infants and those from other human age groups. Twelve different individuals of from four main clusters were selected for further study to reveal the genetic diversity of carbohydrate metabolism-related genes within the same phylogenetics. The animal experiment showed that 3 weeks of oral administration and 1 week after cessation of administration of these strains did not markedly alter the serum routine inflammatory indicators in mice. Furthermore, the administration of these strains did not significantly cause adverse changes in the gut microbiota, as indicated by the α- and β-diversity indexes, relative to the control group (normal diet). Beyond that, FAHBZ9L5 significantly increased the abundance of after 3 weeks and significantly increased the abundance of acetic acid and butyric acid in the host's intestinal tract 3 and 4 weeks after the first administration, respectively, compared with the control group. Corresponding to this, comparative genomic analyses of 12 suggest that FAHBZ9L5-specific genes were rich in ABC transporters and carbohydrate esterase. Combining the results of comparative genomics analyses and animal experiment, it is suggested that the strains containing certain gene clusters contribute to another competitive growth advantage of which facilitates its intestinal carbohydrate metabolism in a host.
从中国不同生境的人、鸡和牛粪便样本中分离出的 88 株菌，在本研究中进行了基因组比较，以评估其多样性。结果发现， 表现出封闭的泛基因组，包含丰富的碳水化合物活性酶（CAZy）糖苷水解酶家族。共鉴定出 30 种糖苷水解酶（GHs）、14 种糖基转移酶（GTs）、13 种碳水化合物结合模块（CBMs）、6 种碳水化合物酯酶（CEs）和 2 种辅助活性（AA）基因家族。具体来说，这表明来自不同年龄组宿主的菌株中，10 种碳水化合物活性酶基因家族（GT51、GH13_32、GH26、GH42、GH121、GH3、AA3、CBM46、CE2 和 CE6）的数量也存在显著差异，特别是在婴儿和其他人类年龄组的菌株之间。从四个主要聚类中选择了 12 个不同个体的 进行进一步研究，以揭示同一系统发育内碳水化合物代谢相关基因的遗传多样性。动物实验表明，这些菌株连续口服 3 周，停药 1 周后，小鼠血清常规炎症指标无明显变化。此外，与对照组（正常饮食）相比，这些菌株的给药并没有显著导致肠道微生物群的不良变化，这表明 α-和 β-多样性指数没有显著变化。除此之外，FAHBZ9L5 在给药 3 周后显著增加了 ，并分别在给药后第 3 周和第 4 周显著增加了宿主肠道内乙酸和丁酸的含量。相应地，对 12 株 的比较基因组分析表明，FAHBZ9L5 特异性基因富含 ABC 转运蛋白和碳水化合物酯酶。将比较基因组分析结果与动物实验相结合，表明含有某些基因簇的菌株有助于 获得另一种竞争生长优势，从而促进其在宿主肠道中的碳水化合物代谢。
