Siuti Nertila, Kelleher Neil L
Department of Chemistry, University of Illinois at Urbana-Champaign, 53 Roger Adams Laboratory, 600 South Matthews Avenue, Urbana, Illinois 61801, USA.
Nat Methods. 2007 Oct;4(10):817-21. doi: 10.1038/nmeth1097.
Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.
自上而下质谱分析是一项新兴技术,它致力于通过完整测量体内存在的蛋白质的翻译后修饰形式,而非测量由蛋白水解产生的肽段来实现这一目标。自上而下技术正开始引起生物学家和质谱分析人员的关注,其主要目标是破解细胞通路中起作用的相互作用网络。在此,我们概述了自上而下质谱分析的近期方法和应用以及对其未来的展望。