School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine 510405, Guangzhou, China.
Molecules. 2009 Dec 21;14(12):5349-61. doi: 10.3390/molecules14125349.
Radix Angelicae Sinensisis (RAS) is one of the most popular traditional Chinese herbal medicines. In the present study, six RAS extracts (i.e., phenolic extract PE, petroleum ether extract PEE, ethyl acetate extract EAE, absolute ethanol extract AEE, 95% ethanol extract 95 EE, and water extract WE) were prepared and their antioxidant activities measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical), ABTS [2,2'-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid diammonium salt)], Reducing power, *O(2-) and lipid peroxidation assays. In general, PE, PEE and EAE had relatively high antioxidant activity, followed by AEE with moderate activity, as compared with 95 EE and WE that had low activity. Their phenolic contents (including total phenolic, ferulic acid, caffeic acid, same as below) were then determined by HPLC or spectrophotometry. The sequence of phenolic contents was roughly identical with that of antioxidant activity. When the values of 1/IC(50) of various antioxidant assays were used to evaluate the level of antioxidant of the RAS extracts, (plot between 1/IC(50) values and phenolic contents), the correlation coefficient (R) ranged from 0.642 to 0.941, with an average value of 0.839. Significant positive correlations demonstrated that the antioxidant effects of RAS might generally be considered a result of the presence of the phenolic compounds, especially ferulic acid and caffeic acid.
当归是一种常用的传统中草药。在本研究中，制备了当归的 6 种提取物（即酚类提取物 PE、石油醚提取物 PEE、乙酸乙酯提取物 EAE、无水乙醇提取物 AEE、95%乙醇提取物 95 EE 和水提取物 WE），并通过 DPPH（1,1-二苯基-2-苦基肼基自由基）、ABTS [2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐]、还原能力、*O(2-) 和脂质过氧化测定法来测定其抗氧化活性。一般来说，PE、PEE 和 EAE 的抗氧化活性相对较高，其次是 AEE 具有中等活性，而 95 EE 和 WE 的活性较低。然后通过 HPLC 或分光光度法测定其酚类含量（包括总酚、阿魏酸、咖啡酸等）。酚类含量的顺序与抗氧化活性大致相同。当使用各种抗氧化测定的 1/IC(50)值来评估当归提取物的抗氧化水平（1/IC(50)值与酚类含量的关系）时，相关系数（R）范围从 0.642 到 0.941，平均值为 0.839。显著的正相关表明，当归的抗氧化作用可能一般归因于酚类化合物的存在，特别是阿魏酸和咖啡酸。
