Chander C L, Desa F M
Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London, UK.
Agents Actions. 1991 Sep;34(1-2):282-4. doi: 10.1007/BF01993303.
We have investigated the effects of estradiol on cartilage breakdown. The model of arthritis used involved the subcutaneous implantation of rat femoral head cartilage (FHCs) into the dorsal region of female mice. The FHCs had been previously wrapped in sterile 5 mg cotton to provoke the growth of granulomatous tissue adjacent to cartilage. Animals were dosed daily, a day after implantation, for 14 days with either 17 beta-estradiol or tamoxifen at different doses. The FHCs were removed and analysed for glycosaminoglycan (GAG) content. The results demonstrated that estradiol accelerated cartilage breakdown in a dose dependent manner, an effect which is blocked by tamoxifen. In addition, estradiol was found to increase cartilage degradation in an in vitro model using FHCs. This effect was enhanced by the addition of fibroblasts.
我们研究了雌二醇对软骨破坏的影响。所使用的关节炎模型包括将大鼠股骨头软骨（FHCs）皮下植入雌性小鼠的背部区域。FHCs先前已用无菌5毫克棉花包裹，以促使软骨相邻的肉芽肿组织生长。动物在植入后一天开始每天给药，持续14天，分别给予不同剂量的17β-雌二醇或他莫昔芬。取出FHCs并分析其糖胺聚糖（GAG）含量。结果表明，雌二醇以剂量依赖性方式加速软骨破坏，他莫昔芬可阻断这一作用。此外，在使用FHCs的体外模型中发现雌二醇会增加软骨降解。添加成纤维细胞可增强这一作用。
