De Brito F B, Moore A R, Holmes M J, Willoughby D A
Department of Experimental Pathology, St Bartholomew's Hospital Medical College, London, UK.
Br J Exp Pathol. 1987 Oct;68(5):675-86.
The encapsulation of intact rat femoral head cartilage or discs of bovine nasal cartilage with cotton-gauze before implantation in the subcutaneous tissues of mice, results in an accelerated loss of cartilage proteoglycan. Loss of proteoglycan from bovine nasal cartilage occurred later than rat femoral head cartilage, but eventually brought about complete dissolution of the cartilage. Freeze-thaw killing of bovine nasal cartilage did not alter the amount of proteoglycan lost. Destruction of both femoral and nasal cartilage was related to the mass of cotton implanted and to the growth of connective tissue within the implanted cotton. Mice previously implanted with femoral head cartilage were able to show enhanced degradation to new implants; this was even greater if the original implants were encapsulated with cotton. Presoaking of cotton-cartilage implants with the non-specific irritant, carrageenan inhibited the breakdown of cartilage. Autoradiographs of 35sulphate pulsed femoral cartilage following implantation with cotton showed reduced incorporation of radiolabel by chondrocytes.
在将完整的大鼠股骨头软骨或牛鼻软骨圆盘植入小鼠皮下组织之前，用纱布包裹，会导致软骨蛋白聚糖加速流失。牛鼻软骨中蛋白聚糖的流失比大鼠股骨头软骨出现得晚，但最终导致软骨完全溶解。牛鼻软骨的冻融处理并未改变蛋白聚糖的流失量。股骨和鼻软骨的破坏与植入棉花的质量以及植入棉花内结缔组织的生长有关。先前植入股骨头软骨的小鼠对新植入物表现出增强的降解能力；如果原始植入物用棉花包裹，这种降解能力会更强。用非特异性刺激物角叉菜胶对棉花 - 软骨植入物进行预浸泡可抑制软骨分解。植入棉花后，用35硫酸盐脉冲处理的股骨软骨的放射自显影片显示软骨细胞对放射性标记物的摄取减少。
