Chen Zigui, Schiffman Mark, Herrero Rolando, Burk Robert D
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD, USA.
J Gen Virol. 2007 Nov;88(Pt 11):2952-2955. doi: 10.1099/vir.0.83178-0.
Complete genomes of HPV102 (8,078 bp) and HPV106 (8,035 bp) were PCR amplified and cloned from cervicovaginal cells of a 49-year-old Hispanic female with reactive changes on her Pap test and a 42-year-old Hispanic female with a Pap test diagnosis of atypical squamous cells of unknown significance (ASCUS), respectively. The nucleotide sequence similarity of the complete L1 open reading frame (ORF) determined that HPV102 and HPV106 are most closely related to HPV83 (84.1 % identity) and HPV90 (83.5 % identity), respectively, placing them in the genital HPV groups, papillomaviruses species alpha3 and alpha15. HPV102 and HPV106 contain five early genes (E6, E7, E1, E2, and E4) and two late genes (L2 and L1), and both lack an E5 ORF. On the basis of phylogenetic analyses and available clinical information, these two novel HPV types expand the heterogeneity of HPVs detected in the lower genital tract.
分别从一名巴氏试验有反应性改变的49岁西班牙裔女性的宫颈阴道细胞以及一名巴氏试验诊断为意义不明确的非典型鳞状细胞（ASCUS）的42岁西班牙裔女性的宫颈阴道细胞中，通过聚合酶链反应（PCR）扩增并克隆出了HPV102（8078碱基对）和HPV106（8035碱基对）的完整基因组。完整的L1开放阅读框（ORF）的核苷酸序列相似性确定，HPV102和HPV106分别与HPV83（同一性为84.1%）和HPV90（同一性为83.5%）关系最为密切，将它们归入生殖器HPV组、乳头瘤病毒α3和α15种。HPV102和HPV106包含五个早期基因（E6、E7、E1、E2和E4）和两个晚期基因（L2和L1），并且两者都缺乏E5 ORF。基于系统发育分析和可用的临床信息，这两种新型HPV类型扩展了在下生殖道中检测到的HPV的异质性。
