Terai M, Burk R D
Department of Microbiology and Immunology, Comprehensive Cancer Center, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461, USA.
Virology. 2001 Jan 5;279(1):109-15. doi: 10.1006/viro.2000.0716.
Molecular diagnosis of human papillomaviruses (HPVs) in cervicovaginal samples reveals a plethora of known and novel HPV genomes. We describe the use of an overlapping PCR method to clone and analyze the complete genome of HPV 84 from cervicovaginal cells obtained from a 21-year-old Caucasian female with a normal Pap smear. The 7948-bp complete nucleotide sequence of HPV 84 was determined from five overlapping PCR products by sequence walking. A BLAST homology search demonstrated that HPV 84 was most closely related to HPV 61 (89%), HPV 72 (86%), and HPV 83 (85%) by nucleotide sequence analysis of the L1 open reading frame, placing it in the HPV genome homology group A3. Previously, this virus had been identified as Pap155. Based on extensive epidemiological data, HPV 84 is a highly prevalent genital papillomavirus primarily detected in normal and HIV-infected women.
对宫颈阴道样本中的人乳头瘤病毒（HPV）进行分子诊断，发现了大量已知和新型的HPV基因组。我们描述了使用重叠PCR方法，从一名巴氏涂片正常的21岁白人女性的宫颈阴道细胞中克隆并分析HPV 84的完整基因组。通过序列步移，从五个重叠PCR产物中确定了HPV 84的7948bp完整核苷酸序列。通过对L1开放阅读框的核苷酸序列分析，BLAST同源性搜索表明，HPV 84与HPV 61（89%）、HPV 72（86%）和HPV 83（85%）关系最为密切，将其归为HPV基因组同源性A3组。此前，这种病毒被鉴定为Pap155。基于广泛的流行病学数据，HPV 84是一种高度流行的生殖器乳头瘤病毒，主要在正常女性和感染HIV的女性中检测到。
