1型和2型糖尿病且无临床大血管并发症患者的内皮功能障碍和氧化应激
Endothelial dysfunction and oxidative stress in type 1 and type 2 diabetic patients without clinical macrovascular complications.
作者信息
De Mattia Giancarlo, Bravi Maria Cristina, Laurenti Oriana, Moretti Arianna, Cipriani Rosalba, Gatti Alessandra, Mandosi Elisabetta, Morano Susanna
机构信息
Department of Internal Medicine, Policlinico Umberto I, Sapienza University, Viale del Policlinico 155, 00161 Rome, Italy.
出版信息
Diabetes Res Clin Pract. 2008 Feb;79(2):337-42. doi: 10.1016/j.diabres.2007.09.005. Epub 2007 Oct 18.
AIMS
To evaluate the relationship between oxidative stress and endothelial dysfunction (ED) in diabetic patients without clinical macrovascular complications.
METHODS
In 27 type 1, 56 type 2 diabetic patients and 35 healthy controls the redox state (GSH, GSSG; enzymatic method), endothelin-1 (ET-1; ELISA) and von Willebrand factor (vWF; ELISA) plasma levels, urinary vascular endothelial growth factor (VEGF; ELISA) were measured.
RESULTS
Decreased GSH levels (p<0.05, type 1 and type 2), GSH/GSSG ratio (p<0.05 type 1, p<0.001 type 2) and elevated vWF levels (p<0.001, type 1 and type 2) were observed in diabetic patients in comparison with controls. A negative correlation between GSH and vWF (p<0.02 and p<0.001, in type 1 and type 2, respectively) and GSH and BMI (p<0.02 in type 1 and type 2) was observed. ET-1 was positively correlated to age (p<0.05) and diabetes duration (p<0.03) in type 1, while vWF was correlated to systolic blood pressure (p<0.05) in type 2 diabetic patients. Urinary VEGF was higher in type 2 (p<0.05) in comparison with type 1 diabetic patients and was correlated to glycemia (p<0.05) and systolic blood pressure (p<0.05).
CONCLUSIONS
These data might indicate that markers of oxidative stress and ED are altered in diabetic patients without clinical macrovascular complications.
目的
评估无临床大血管并发症的糖尿病患者氧化应激与内皮功能障碍（ED）之间的关系。
方法
对27例1型糖尿病患者、56例2型糖尿病患者和35名健康对照者测定氧化还原状态（谷胱甘肽、氧化型谷胱甘肽；酶法）、血浆内皮素-1（ET-1；酶联免疫吸附测定法）和血管性血友病因子（vWF；酶联免疫吸附测定法）水平以及尿血管内皮生长因子（VEGF；酶联免疫吸附测定法）。
结果
与对照组相比，糖尿病患者的谷胱甘肽水平降低（1型和2型，p<0.05）、谷胱甘肽/氧化型谷胱甘肽比值降低（1型，p<0.05；2型，p<0.001）以及vWF水平升高（1型和2型，p<0.001）。观察到谷胱甘肽与vWF之间呈负相关（1型和2型分别为p<0.02和p<0.001）以及谷胱甘肽与体重指数之间呈负相关（1型和2型均为p<0.02）。在1型糖尿病中，ET-1与年龄呈正相关（p<0.05）以及与糖尿病病程呈正相关（p<0.03），而在2型糖尿病患者中，vWF与收缩压呈正相关（p<0.05）。与1型糖尿病患者相比，2型糖尿病患者的尿VEGF更高（p<0.05），并且与血糖（p<0.05）和收缩压（p<0.05）相关。
结论
这些数据可能表明，无临床大血管并发症的糖尿病患者氧化应激和内皮功能障碍标志物发生了改变。
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