鸟苷酸环化酶的阳离子依赖性催乳素激活作用。

Cation-dependent prolactin activation of guanylate cyclase.

作者信息

Vesely D L

出版信息

Biochem Biophys Res Commun. 1984 Sep 28;123(3):1084-90. doi: 10.1016/s0006-291x(84)80244-2.

Abstract

Prolactin enhanced guanylate cyclase [E.C.4.6.1.2] two- to threefold in ovary, testis, mammary gland, liver and kidney. Dose response relationships revealed that maximal activation of this enzyme was at a concentration of one nanomolar and that increasing prolactin's concentration to the millimolar range caused no further increase in activity. There was an absolute cation requirement for prolactin's enhancement of guanylate cyclase. Calcium or manganese allowed prolactin to increase guanylate cyclase activity. Greater enhancement of this enzyme's activity by prolactin was observed when manganese was the co-factor. The data in this investigation suggest that guanylate cyclase may play a role in the mechanism of action of prolactin.

摘要

催乳素可使卵巢、睾丸、乳腺、肝脏和肾脏中的鸟苷酸环化酶（[E.C.4.6.1.2]）活性增强两到三倍。剂量反应关系表明，该酶的最大激活浓度为一纳摩尔，将催乳素浓度提高到毫摩尔范围不会使活性进一步增加。催乳素增强鸟苷酸环化酶活性绝对需要阳离子。钙或锰可使催乳素增加鸟苷酸环化酶活性。当以锰作为辅助因子时，催乳素对该酶活性的增强作用更明显。本研究中的数据表明，鸟苷酸环化酶可能在催乳素的作用机制中发挥作用。