Quan Timothy E, Bucala Richard
Methods Mol Med. 2007;135:423-34. doi: 10.1007/978-1-59745-401-8_28.
Fibrocytes circulate in the peripheral blood, produce collagen and other matrix proteins, and express cell surface markers indicative of a hematopoetic origin distinguishing them from fibroblasts. Circulating fibrocytes were first identified in 1994 in a model system of wound repair, and defined by their growth characteristics and unique surface phenotype. The methods currently employed for the isolation, growth, and characterization of peripheral blood fibrocytes rely on the entry of "fibroblast-like" cells into wound chambers, or the derivation of "fibroblast-like" cells from the buffy coat of peripheral blood obtained from different mammalian species. In this protocol, we culture fibrocytes from the mononuclear cells of peripheral blood and harvest the cultured cells for flow cytometry analysis.
纤维细胞在外周血中循环，产生胶原蛋白和其他基质蛋白，并表达表明其造血起源的细胞表面标志物，这使其有别于成纤维细胞。循环纤维细胞于1994年在伤口修复模型系统中首次被鉴定，并根据其生长特性和独特的表面表型来定义。目前用于分离、培养和鉴定外周血纤维细胞的方法依赖于“成纤维细胞样”细胞进入伤口腔室，或从不同哺乳动物物种的外周血血沉棕黄层中衍生出“成纤维细胞样”细胞。在本实验方案中，我们从外周血单核细胞中培养纤维细胞，并收获培养的细胞用于流式细胞术分析。
