Gómez-Sjöberg Rafael, Leyrat Anne A, Pirone Dana M, Chen Christopher S, Quake Stephen R
Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, Stanford, California 94305, USA.
Anal Chem. 2007 Nov 15;79(22):8557-63. doi: 10.1021/ac071311w. Epub 2007 Oct 23.
There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with time-lapse microscopy. Using this device, we perform the first quantitative measurements of the influence of transient stimulation schedules on the proliferation, osteogenic differentiation, and motility of human primary mesenchymal stem cells.
干细胞生物学的活跃以及系统生物学的出现，推动了对自动化定量细胞培养技术需求的不断增长。我们基于微流控芯片构建了一个全自动细胞培养筛选系统，该系统可在96个独立培养室中创建任意培养基配方，并能维持细胞活力数周。可根据细胞接种密度、培养基成分和补料时间表定制各个培养条件，并且每个培养室都通过延时显微镜进行成像。利用该设备，我们首次对瞬时刺激方案对人原代间充质干细胞增殖、成骨分化和运动性的影响进行了定量测量。
