Velazquez C, Navarro M, Acosta A, Angulo A, Dominguez Z, Robles R, Robles-Zepeda R, Lugo E, Goycoolea F M, Velazquez E F, Astiazaran H, Hernandez J
Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.
J Appl Microbiol. 2007 Nov;103(5):1747-56. doi: 10.1111/j.1365-2672.2007.03409.x.
To evaluate the antibacterial and free-radical scavenging (FRS) activities of propolis collected from three different areas of Sonoran Desert in northwestern Mexico [Pueblo de Alamos (PAP), Ures (UP) and Caborca (CP)].
The antibacterial and FRS activities of Sonoran propolis were determined by the broth microdilution method and the DPPH (1,1-diphenyl-2-picrylhydracyl) assay, respectively. Propolis samples had antibacterial activity against only Gram-positive bacteria. The UP sample showed the highest antibacterial activity against Staphylococcus aureus [minimal inhibitory concentration (MIC) 100 microg ml(-1)] in a concentration-dependent manner (UP > CP > PAP). Caffeic acid phenethyl ester (CAPE), a UP propolis constituent, had very high growth-inhibitory activity towards Gram-positive bacteria, particularly against S. aureus (MIC 0.1 mmol l(-1)). To our knowledge, this is the first study showing a strong antibacterial activity of CAPE against S. aureus. Additionally, propolis CP exhibited high FRS activity (86% +/- 0.3 at 100 microg ml(-1)) comparable with those of the reference antioxidants vitamin C (87.4% +/- 1.7 at 70 micromol l(-1)) and BHT (66.07% +/- 0.76 at 140 micromol l(-1)). The propolis compounds CAPE and rutin showed high FRS activity (90.4% +/- 0.2 and 88.5% +/- 0.8 at 70 micromol l(-1), respectively).
Sonoran propolis UP and CAPE had strong antibacterial activity against S. aureus. In addition, propolis CP showed potent FRS activity comparable with those of vitamin C and BHT.
The strong antibacterial and antioxidant properties of Sonoran propolis and some of its constituents support further studies on the clinical applications of this natural bee product against S. aureus and several oxidative damage-related diseases.
评估从墨西哥西北部索诺兰沙漠三个不同地区（阿拉莫斯镇（PAP）、乌雷斯（UP）和卡博卡（CP））采集的蜂胶的抗菌和自由基清除（FRS）活性。
分别采用肉汤微量稀释法和DPPH（1,1 - 二苯基 - 2 - 苦基肼）法测定索诺兰蜂胶的抗菌和FRS活性。蜂胶样品仅对革兰氏阳性菌有抗菌活性。UP样品对金黄色葡萄球菌表现出最高的抗菌活性[最低抑菌浓度（MIC）为100微克/毫升]，呈浓度依赖性（UP > CP > PAP）。咖啡酸苯乙酯（CAPE）是UP蜂胶的一种成分，对革兰氏阳性菌具有非常高的生长抑制活性，尤其是对金黄色葡萄球菌（MIC为0.1毫摩尔/升）。据我们所知，这是第一项显示CAPE对金黄色葡萄球菌具有强抗菌活性的研究。此外，蜂胶CP表现出高FRS活性（100微克/毫升时为86% ± 0.3），与参考抗氧化剂维生素C（70微摩尔/升时为87.4% ± 1.7）和丁基羟基甲苯（BHT，140微摩尔/升时为66.07% ± 0.76）相当。蜂胶成分CAPE和芦丁表现出高FRS活性（70微摩尔/升时分别为90.4% ± 0.2和88.5% ± 0.8）。
索诺兰蜂胶UP和CAPE对金黄色葡萄球菌具有强抗菌活性。此外，蜂胶CP表现出与维生素C和BHT相当的强FRS活性。
索诺兰蜂胶及其一些成分的强抗菌和抗氧化特性支持对这种天然蜂产品针对金黄色葡萄球菌和几种与氧化损伤相关疾病的临床应用进行进一步研究。
