Teng C S, Teng C T
Biochem J. 1980 May 15;188(2):313-9. doi: 10.1042/bj1880313.
The response of ornithine decarboxylase activity to hormones in the embryonic left ovary was measured throughout the stages of development. During the early stage of ovarian development (9th day of incubation), the ornithine decarboxylase activity (in terms of pmol CO(2)/30min per mg of protein) was high (766); it decreased from the 10th to the 12th day (575-239), increased slightly from the 13th to the 15th day (306) and finally fell to a low value (192-20) from the 18th day of development to birth. Administration of an optimal dose of oestrogen to the 9-10-day embryo stimulated the ovarian ornithine decarboxylase activity by 48-53%. If the same dose of oestrogen was administered to the 15-18-day embryo, the ovarian enzyme activity was suppressed by 32-43%. This biphasic response to oestrogen for enzyme induction is characteristic of the developing ovary and is not observed in other genital organs of the chick. In the early developmental stage (9-10th day) testosterone has no effect on ovarian ornithine decarboxylase activity, but in the late stage testosterone inhibits the activity by 41%. Organ culture techniques have been used to test the ovarian response to lutropin (luteinizing hormone). Lutropin stimulated ornithine decarboxylase activity by approx. 99-155% in the ovary of the early embryonic stage (10-13th day), and by 175-200% in the ovary of the late embryonic stage (15-18th day). The alteration in enzyme activity in the ovary as assayed in vitro during development is not due to the effect of the size of the endogenous ornithine pool. The relationship of ornithine decarboxylase activity to the morphological and biochemical changes in the developing ovary is discussed.
在整个胚胎发育阶段，对胚胎左侧卵巢中鸟氨酸脱羧酶活性对激素的反应进行了测定。在卵巢发育的早期阶段（孵化第9天），鸟氨酸脱羧酶活性（以每毫克蛋白质每分钟产生的pmol CO₂计）较高（766）；从第10天到第12天活性下降（575 - 239），从第13天到第15天略有增加（306），最终从发育第18天到出生时降至较低值（192 - 20）。给9 - 10天的胚胎注射最佳剂量的雌激素可使卵巢鸟氨酸脱羧酶活性提高48 - 53%。如果给15 - 18天的胚胎注射相同剂量的雌激素，卵巢酶活性会被抑制32 - 43%。这种对雌激素诱导酶的双相反应是发育中卵巢的特征，在鸡的其他生殖器官中未观察到。在发育早期阶段（第9 - 10天），睾酮对卵巢鸟氨酸脱羧酶活性没有影响，但在后期阶段睾酮会使活性降低41%。已使用器官培养技术来测试卵巢对促黄体素（促黄体生成素）的反应。促黄体素可使早期胚胎阶段（第10 - 13天）卵巢中的鸟氨酸脱羧酶活性提高约99 - 155%，在晚期胚胎阶段（第15 - 18天）卵巢中的活性提高175 - 200%。在发育过程中体外测定的卵巢中酶活性的变化并非由于内源性鸟氨酸池大小的影响。本文讨论了鸟氨酸脱羧酶活性与发育中卵巢的形态和生化变化之间的关系。
