Song Wen-Liang, Lawson John A, Wang Miao, Zou Helen, FitzGerald Garret A
Institute for Translational Medicine and Therapeutics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Methods Enzymol. 2007;433:51-72. doi: 10.1016/S0076-6879(07)33003-6.
A robust method for the routine quantitation of a selected group of urinary eicosanoid metabolites of interest to cardiovascular research in human and mouse is described and discussed. Included are the addition of stable isotope-labeled internal standards, solid phase extraction, and quantitation by liquid chromatography/tandem mass spectrometry using selected reaction monitoring (SRM) techniques.
本文描述并讨论了一种用于常规定量检测人类和小鼠尿液中一组对心血管研究有意义的特定类花生酸代谢物的可靠方法。该方法包括添加稳定同位素标记的内标、固相萃取，以及使用选择反应监测（SRM）技术通过液相色谱/串联质谱法定量。
