Culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers.

In preliminary studies, a system for the propagation of respiratory syncytial virus (RSV) using cytodex 3 microcarriers was established. Optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/L with a cell inoculum density of 4 X 10(4) cells/ml tissue culture medium. Under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. Maximum yield of virus antigen was achieved after 8 days culture.