Nara Asha, Chandra Prakash, Anandakrishna Latha
Postgraduate Student, Department of Pedodontics and Preventive Dentistry, MS Ramiah Dental College and Hospital Bengaluru, Karnataka, India.
Professor, Department of Pedodontics and Preventive Dentistry, MS Ramiah Dental College and Hospital Bengaluru, Karnataka, India.
Int J Clin Pediatr Dent. 2010 Jan-Apr;3(1):21-5. doi: 10.5005/jp-journals-10005-1049. Epub 2010 Apr 15.
The present study sought to compare the antimicrobial efficacy of 3% NaOCl, Biopure MTAD (Tulsa Dentsply, Tulsa, OK) and Brazilian ethanolic extract of propolis (EEP) against Enterococcus faecalis (E. faecalis).
The study utilized 55 extracted human permanent teeth with single root canal. The samples were decoronated, instrumented and sterilized. The teeth were infected with E faecalis for 48 hours. The teeth were divided randomly into 3 groups according to the irrigants used and kept in contact with the respective irrigant for 5 minutes. All the samples were incubated in brain heart infusion (BHI) broth for 96 hours. Disinfection of the samples was determined based on presence or absence of turbidity in the BHI broth 96 hours later. Statistical analysis was done using Chi-square test.
All the samples treated with MTAD showed complete absence of turbidity, while all the 15 teeth treated with propolis showed presence of turbidity, 8 out of 15 teeth treated with NaOCl showed presence of turbidity. Statistical analysis of the data using chi-square test showed significant difference between the groups (P < 0.05).
The study concluded that MTAD was more effective than 3% NaOCl and propolis against E. faecalis.
本研究旨在比较3%次氯酸钠、生物纯MTAD(登士柏国际公司,塔尔萨,俄克拉何马州)和巴西蜂胶乙醇提取物(EEP)对粪肠球菌的抗菌效果。
本研究使用了55颗单根管的拔除人类恒牙。样本进行去冠、预备和消毒处理。将牙齿感染粪肠球菌48小时。根据所使用的冲洗剂将牙齿随机分为3组,并与相应的冲洗剂接触5分钟。所有样本在脑心浸液(BHI)肉汤中孵育96小时。96小时后,根据BHI肉汤中是否有浑浊来确定样本的消毒情况。使用卡方检验进行统计分析。
所有用MTAD处理的样本均无浑浊,而所有15颗用蜂胶处理的牙齿均有浑浊,15颗用次氯酸钠处理的牙齿中有8颗有浑浊。使用卡方检验对数据进行统计分析,结果显示各组之间存在显著差异(P < 0.05)。
该研究得出结论,MTAD对粪肠球菌的抗菌效果优于3%次氯酸钠和蜂胶。