• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

核酸片段的快速高效电洗脱

Quick and high-efficiency electroelution of nucleic acid fragments.

作者信息

Pai S R, Bird R C

机构信息

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849.

出版信息

Genet Anal Tech Appl. 1991 Nov;8(7):214-6. doi: 10.1016/1050-3862(91)90015-j.

DOI:10.1016/1050-3862(91)90015-j
PMID:1797004
Abstract

We describe a quick, simple, and inexpensive technique for the electroelution of nucleic acid fragments that provides a high yield of DNA by using common laboratory components. The quantity of buffer used for the recovery of nucleic acid fragments is relatively small, the quality of the DNA recovered is relatively high, and more than one electroelution can be carried out at the same time.

摘要

我们描述了一种快速、简单且廉价的核酸片段电洗脱技术,该技术使用常见的实验室组件就能实现高产量的DNA提取。用于回收核酸片段的缓冲液用量相对较少,回收的DNA质量相对较高,并且可以同时进行多次电洗脱。

相似文献

1
Quick and high-efficiency electroelution of nucleic acid fragments.核酸片段的快速高效电洗脱
Genet Anal Tech Appl. 1991 Nov;8(7):214-6. doi: 10.1016/1050-3862(91)90015-j.
2
A simple technique for electroelution of DNA from environmental samples.一种从环境样本中电洗脱DNA的简单技术。
Biotechniques. 1991 Dec;11(6):724, 726-8.
3
Simultaneous electroelution and concentration of DNA fragments from agarose gels.从琼脂糖凝胶中同时进行DNA片段的电洗脱和浓缩。
Anal Biochem. 1988 Feb 15;169(1):194-6. doi: 10.1016/0003-2697(88)90273-4.
4
Rapid recovery of DNA from agarose gel slices by coupling electroelution with monolithic SPE.通过将电洗脱与整体式固相萃取相结合从琼脂糖凝胶切片中快速回收DNA
Electrophoresis. 2009 Jun;30(12):2110-6. doi: 10.1002/elps.200800777.
5
Rapid and inexpensive micro-electroelution of nucleic acid and protein from agarose and polyacrylamide gels.从琼脂糖凝胶和聚丙烯酰胺凝胶中快速且低成本地进行核酸和蛋白质的微量电洗脱
Biotechniques. 1992 Sep;13(3):368.
6
A simple, inexpensive electroelution device for the recovery of nucleic acid fragments from agarose gels.
Biotechniques. 1991 Feb;10(2):159-60.
7
Electroeluting DNA fragments.电洗脱DNA片段。
J Vis Exp. 2010 Sep 5(43):2136. doi: 10.3791/2136.
8
Efficient, small scale electroelution of high molecular weight DNA from agarose gels by a miniature vertical electrophoresis cell.使用微型垂直电泳槽从琼脂糖凝胶中高效小规模电洗脱高分子量DNA
Electrophoresis. 1991 Apr;12(4):317-20. doi: 10.1002/elps.1150120417.
9
Extraction of nucleic acids from agarose gel--a quantitative and qualitative comparison of four different methods.从琼脂糖凝胶中提取核酸——四种不同方法的定量和定性比较
Prep Biochem. 1990;20(2):123-35. doi: 10.1080/00327489008050184.
10
Electroelution of DNA and protein from polyacrylamide and agarose gels.从聚丙烯酰胺凝胶和琼脂糖凝胶中电洗脱DNA和蛋白质。
Biochem Int. 1987 Oct;15(4):687-91.

引用本文的文献

1
Adaptation of Xanthobacter autotrophicus GJ10 to bromoacetate due to activation and mobilization of the haloacetate dehalogenase gene by insertion element IS1247.自养黄色杆菌GJ10对溴乙酸的适应性是由于插入元件IS1247激活并调动了卤乙酸脱卤酶基因。
J Bacteriol. 1995 Mar;177(5):1348-56. doi: 10.1128/jb.177.5.1348-1356.1995.