核酸片段的快速高效电洗脱
Quick and high-efficiency electroelution of nucleic acid fragments.
作者信息
Pai S R, Bird R C
机构信息
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849.
出版信息
Genet Anal Tech Appl. 1991 Nov;8(7):214-6. doi: 10.1016/1050-3862(91)90015-j.
PMID:1797004
Abstract
We describe a quick, simple, and inexpensive technique for the electroelution of nucleic acid fragments that provides a high yield of DNA by using common laboratory components. The quantity of buffer used for the recovery of nucleic acid fragments is relatively small, the quality of the DNA recovered is relatively high, and more than one electroelution can be carried out at the same time.
摘要
我们描述了一种快速、简单且廉价的核酸片段电洗脱技术，该技术使用常见的实验室组件就能实现高产量的DNA提取。用于回收核酸片段的缓冲液用量相对较少，回收的DNA质量相对较高，并且可以同时进行多次电洗脱。
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