Rao E V, Ramana K S
Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam, India.
Carbohydr Res. 1991 Sep 18;217:163-70. doi: 10.1016/0008-6215(91)84126-y.
Extraction of the green seaweed Chaetomorpha anteninna with water, followed by fractionation using the copper complex, gave a sulphated heteropolysaccharide, [alpha]D27 +84 degrees, that contained arabinose (57.7%), galactose (38.5%), rhamnose (3.8%), and sulphate (11.9%). Application of methylation analysis, periodate oxidation, and partial acid hydrolysis indicated a branched structure with 4-linked arabinose, 4- and 3-linked galactose, and 4-linked rhamnose residues. Both the arabinose and galactose residues carried branches. Desulphation and analysis of the polymeric product showed that sulphate groups were located at C-2 of some of the interior arabinose residues, C-4 of some of the non-reducing-end arabisoyl groups, and C-3 of the non-reducing-end galactosyl groups.
用水提取绿藻仙菜，然后用铜络合物进行分级分离，得到一种硫酸化杂多糖，[α]D27 +84°，其含有阿拉伯糖（57.7%）、半乳糖（38.5%）、鼠李糖（3.8%）和硫酸盐（11.9%）。甲基化分析、高碘酸盐氧化和部分酸水解表明其具有分支结构，含有4-连接的阿拉伯糖、4-和3-连接的半乳糖以及4-连接的鼠李糖残基。阿拉伯糖和半乳糖残基均带有分支。对聚合物产物进行脱硫酸化和分析表明，硫酸基团位于一些内部阿拉伯糖残基的C-2位、一些非还原末端阿拉伯糖酰基的C-4位以及非还原末端半乳糖基的C-3位。
