Ito Y, Kajihara M, Imanishi Y
Department of Polymer Chemistry, Kyoto University, Japan.
J Biomed Mater Res. 1991 Nov;25(11):1325-37. doi: 10.1002/jbm.820251102.
Materials to enhance cell adhesion were synthesized by surface integration of peptide, Arg-Gly-Asp-Ser(RGDS), which is an active-site sequence of cell-adhesive proteins. Polystyrene film was glow-discharged and graft-copolymerized with acrylic acid. Then the peptide was immobilized to the poly(acrylic acid) grafts by using water-soluble carbodiimide. The cell-adhesive activity of the RGDS-immobilized film increased with increasing amount of immobilized peptide, and approached the activity of fibronectin(FN)-immobilized film. The RGDS-immobilized film was more stable against heat treatment and pH variation than the FN-immobilized film. In addition, the RGDS-immobilized film enhanced cell growth more strongly than the FN-immobilized film.
通过对细胞黏附蛋白的活性位点序列——肽段精氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）进行表面整合，合成了增强细胞黏附的材料。对聚苯乙烯薄膜进行辉光放电，并使其与丙烯酸接枝共聚。然后使用水溶性碳二亚胺将该肽固定到聚（丙烯酸）接枝上。固定有RGDS的薄膜的细胞黏附活性随固定肽量的增加而增加，并接近固定有纤连蛋白（FN）的薄膜的活性。与固定有FN的薄膜相比，固定有RGDS的薄膜对热处理和pH变化更稳定。此外，固定有RGDS的薄膜比固定有FN的薄膜更能强烈地促进细胞生长。
