Das Siddhartha, Gurav Sandeep, Soni Vivek, Ingle Arvind, Mohanty Bhabani S, Chaudhari Pradip, Bendale Kiran, Dholam Kanchan, Bellare Jayesh R
1Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, 400076 India.
2Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, 400076 India.
Tissue Eng Regen Med. 2018 Jan 19;15(2):231-247. doi: 10.1007/s13770-017-0106-6. eCollection 2018 Apr.
A titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and hence results in enhanced osseointegration of the implant. The osteogenic nanofibrous coating (ONFC) consists of poly-caprolactone, gelatin, nano-sized hydroxyapatite, dexamethasone, ascorbic acid and beta-glycerophosphate. ONFC exhibits optimum mechanical properties to support mesenchymal stem cells and steer their osteogenic differentiation. ONFC was subjected to various characterization tests like scanning electron microscopy, Fourier-transform infrared spectroscopy, x-ray diffractometry, thermal degradation, biomineralization, mechanical properties, wettability and proliferation assay. In pre-clinical animal trials, the coated implant showed enhanced new bone formation when placed in the tibia of rabbit. This novel approach toward implant bone integration holds significant promise for its easy and economical coating thus marking the beginning of new era of electrospun osteogenic nanofibrous coated bone implants.
当钛植入物表面涂覆有可生物降解、高度多孔的成骨纳米纤维涂层时，已显示出增强的内在骨诱导和骨传导特性。这种涂层模仿细胞外基质，导致植入物周围微环境中存在的干细胞分化为成骨细胞，从而增强植入物的骨整合。成骨纳米纤维涂层（ONFC）由聚己内酯、明胶、纳米级羟基磷灰石、地塞米松、抗坏血酸和β-甘油磷酸组成。ONFC具有最佳的机械性能，以支持间充质干细胞并引导其成骨分化。ONFC进行了各种表征测试，如扫描电子显微镜、傅里叶变换红外光谱、X射线衍射、热降解、生物矿化、机械性能、润湿性和增殖测定。在临床前动物试验中，当将涂覆的植入物置于兔子胫骨中时，显示出新骨形成增强。这种植入物骨整合的新方法因其简便且经济的涂层而具有重大前景，从而标志着电纺成骨纳米纤维涂层骨植入物新时代的开始。
