Hirano Y, Kando Y, Hayashi T, Goto K, Nakajima A
Department of Applied Chemistry, Osaka Institute of Technology, Japan.
J Biomed Mater Res. 1991 Dec;25(12):1523-34. doi: 10.1002/jbm.820251209.
Tetrapeptides, Arg-Gly-Asp-Ser (RGDS), Arg-Gly-Asp-Val (RGDV), and Arg-Gly-Asp-Thr (RGDT), present in the cell-attachment domain of fibronectin, vitronectin, and collagen, respectively, were synthesized by using an improved liquid-phase procedure. Bioactivities of RGD and RGDX (X = S, V, and T) as cell recognition determinants were investigated by two methods to evaluate interactions of these oligopeptides with L-929 fibroblast cells originating in mouse epithelia. In the first method, these oligopeptides were immobilized to ethylene-acrylic acid copolymer (EAA) film, and the cell-attachment activity of the immobilized film was measured. In the second method, interaction of oligopeptides with the cells was evaluated by measuring the cell inhibition caused by oligopeptides. It was found that RGD and RGDX exhibit remarkable cell-attachment activity, and the activity of oligopeptides depends on X.
四肽，即分别存在于纤连蛋白、玻连蛋白和胶原蛋白细胞附着结构域中的精氨酸-甘氨酸-天冬氨酸-丝氨酸（RGDS）、精氨酸-甘氨酸-天冬氨酸-缬氨酸（RGDV）和精氨酸-甘氨酸-天冬氨酸-苏氨酸（RGDT），采用改进的液相法合成。通过两种方法研究了RGD和RGDX（X = S、V和T）作为细胞识别决定簇的生物活性，以评估这些寡肽与源自小鼠上皮的L-929成纤维细胞的相互作用。在第一种方法中，将这些寡肽固定在乙烯-丙烯酸共聚物（EAA）膜上，并测量固定膜的细胞附着活性。在第二种方法中，通过测量寡肽引起的细胞抑制来评估寡肽与细胞的相互作用。结果发现，RGD和RGDX表现出显著的细胞附着活性，且寡肽的活性取决于X。
