Lorenzo-Morales Jacob, Coronado-Alvarez Nieves, Martínez-Carretero Enrique, Maciver Sutherland K, Valladares Basilio
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, University of La Laguna, La Laguna, Canary Islands, Spain.
Am J Trop Med Hyg. 2007 Oct;77(4):753-6.
We surveyed 236 potentially pathogenic Acanthamoeba strains, isolated from water sources in the Canary Islands, for the presence of human adenoviruses (HAdV) using a polymerase chain reaction (PCR)-based typing assay. A total of 34 of these strains were found to be positive for adenovirus belonging to four different HAdV serotypes (HAdV-1, 2, 8, and 37). We found that HAdV-2 was the most frequently encountered serotype amongst the Acanthamoeba strains, and their identification was confirmed by a nested PCR specific for this serotype. We showed that Acanthamoeba genotype T4 was highly associated with serotype HAdV-2, whereas Acanthamoeba genotype T3 was most often associated with adenovirus serotypes related to ocular diseases. Based on these data, we suggest that Acanthamoeba should be considered as a potential reservoir and perhaps even a transmitter of adenoviruses to human and other secondary hosts.
我们使用基于聚合酶链反应（PCR）的分型检测方法，对从加那利群岛水源中分离出的236株潜在致病性棘阿米巴菌株进行了检测，以确定是否存在人腺病毒（HAdV）。结果发现，其中共有34株菌株的腺病毒检测呈阳性，这些腺病毒属于四种不同的HAdV血清型（HAdV-1、2、8和37）。我们发现，HAdV-2是棘阿米巴菌株中最常出现的血清型，通过针对该血清型的巢式PCR证实了对它们的鉴定。我们还发现，棘阿米巴基因型T4与血清型HAdV-2高度相关，而棘阿米巴基因型T3最常与眼部疾病相关的腺病毒血清型有关。基于这些数据，我们建议应将棘阿米巴视为腺病毒的潜在储存宿主，甚至可能是向人类和其他中间宿主传播腺病毒的媒介。
