Gupta Vinod K, Singh Ashok K, Gupta Barkha
Department of Chemistry, Indian Institute of Technology, Roorkee, India.
Comb Chem High Throughput Screen. 2007 Aug;10(7):560-70. doi: 10.2174/138620707782152416.
The construction and characterization of potentiometric membrane electrodes are described for the quantification of alfuzosin, a drug used in a mono- and combined therapy of benign prostatic hyperplasia (BPH). The membranes of these electrodes consist of alfuzosin hydrochloride-tetraphenyl borate, (Az-TPB), chlorophenyl borate (Az-ClPB), and phosphotungstate (Az(3)-PT) ion associations as molecular recognition reagent dispersed in PVC matrix with dioctylpthalate as plasticizer. The performance characteristics of these electrodes, which were evaluated according to IUPAC recommendations, revealed a fast, stable and liner response for alfuzosin over the concentration ranges of 8.3 x 10(-6) to 1.0 x 10(-2) M, 3.8 x 10(-6) to 1.0 x 10(-2) M, 7.5 x 10(-7) to 1.0 x 10(-2) M AzCl with cationic slopes of 57.0, 56.0 and 58.5 mV/decade, respectively. The solubility product of the ion-pair and the formation constant of the precipitation reaction leading to the ion-pair formation were determined conductometrically. The electrodes, fully characterized in terms of composition, life span and usable pH range, were applied to the potentiometric determination of alfuzosin hydrochloride ion in different pharmaceutical preparations and biological fluids without any interference from excipients or diluents commonly used in drug formulations. The potentiometric method was also used in the determination of alfuzosin hydrochloride in pharmaceutical preparations in four batches with different expiration dates. Validation of the method showed suitability of the proposed electrodes for use in the quality control assessment of alfuzosin hydrochloride. This potentiometric method offers the advantages of high-throughput determination, simplicity, accuracy, automation feasibility, and applicability to turbid and colored sample solutions.
描述了用于定量阿夫唑嗪的电位膜电极的构建和表征，阿夫唑嗪是一种用于良性前列腺增生（BPH）单一疗法和联合疗法的药物。这些电极的膜由盐酸阿夫唑嗪 - 四苯基硼酸盐（Az - TPB）、氯苯基硼酸盐（Az - ClPB）和磷钨酸盐（Az(3)-PT）离子缔合物作为分子识别试剂组成，分散在以邻苯二甲酸二辛酯为增塑剂的PVC基质中。根据国际纯粹与应用化学联合会（IUPAC）的建议对这些电极的性能特征进行评估，结果表明在8.3×10(-6)至1.0×10(-2) M、3.8×10(-6)至1.0×10(-2) M、7.5×10(-7)至1.0×10(-2) M阿夫唑嗪氯的浓度范围内，对阿夫唑嗪有快速、稳定且线性的响应，阳离子斜率分别为57.0、56.0和58.5 mV/十年。通过电导法测定了离子对的溶度积和导致离子对形成的沉淀反应的形成常数。这些电极在组成、寿命和可用pH范围方面得到了充分表征，并应用于电位法测定不同药物制剂和生物流体中的盐酸阿夫唑嗪离子，不受药物制剂中常用辅料或稀释剂的任何干扰。电位法还用于测定四批不同有效期的药物制剂中的盐酸阿夫唑嗪。该方法的验证表明所提出的电极适用于盐酸阿夫唑嗪的质量控制评估。这种电位法具有高通量测定、简单、准确、自动化可行性以及适用于浑浊和有色样品溶液的优点。
