Tagawa Miho, Shohda Koh-ichiroh, Fujimoto Kenzo, Sugawara Tadashi, Suyama Akira
Department of Life Sciences and Institute of Physics, Graduate School of Arts and Sciences, The University of Tokyo, Japan.
Nucleic Acids Res. 2007;35(21):e140. doi: 10.1093/nar/gkm872. Epub 2007 Nov 3.
Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostructures created by self-assembly of the DXAB tiles before and after photoligation have been visualized by high-resolution, tapping mode atomic force microscopy in buffer. The improvement of the heat tolerance of 2D DNA arrays was confirmed by heating and visualizing the DNA nanostructures. The heat-resistant DNA arrays may expand the potential of DNA as functional materials in biotechnology and nanotechnology.
模板导向的DNA光连接已应用于一种构建耐热二维（2D）DNA阵列的方法，该阵列可作为功能性生物分子和纳米电子元件自下而上组装的支架。DNA双交叉AB交错（DXAB）瓦片通过无酶模板导向的光连接共价连接，这使得在极其狭小的空间以及酶无法高效发挥作用的缓冲条件下能够进行特异性连接反应。光连接前后由DXAB瓦片自组装产生的DNA纳米结构已通过缓冲液中高分辨率的轻敲模式原子力显微镜进行了可视化观察。通过对DNA纳米结构进行加热和可视化观察，证实了二维DNA阵列耐热性的提高。这种耐热DNA阵列可能会拓展DNA作为生物技术和纳米技术中功能材料的潜力。
