Miguel Amparo, Orero Maite, Simon Ramon, Collado Rosa, Perez Pedro L, Pacios Alejandro, Iglesias Rosario, Martinez Antonio, Carbonell Felix
Servicio de Hematologia, Consorcio Hospital General Universitario, Valencia, Spain.
Lab Hematol. 2007;13(3):98-102. doi: 10.1532/LH96.07011.
The automated hematology cell analyzer Coulter LH 750 (Beckman Coulter, Brea, CA, USA) uses a combination of 3 measurements-volume, conductivity, and scatter-to identify cells, but it could take advantage of these parameters to evaluate their morphologic changes. The neutrophil mean cell volume (MNEV), mean cell conductivity (MNEC), and mean cell scatter (MNES) were evaluated in 54 patients with myelodysplastic syndrome (MDS), 18 with chronic myelomonocytic leukemia (CMML), and 59 healthy controls. MNES and MNEC in the MDS group including all subtypes (World Health Organization classification) and in the CMML patients were significantly lower than the control group. MNES in MDS and CMML correlated well with the cytoplasmic hypogranularity evaluated by microscopic observation (P = .01). The value of MNES and MNEC as screening parameters in the neutrophil dysplasia evaluation showed, for this study, a sensitivity of 71.8% with a specificity of 86.4% (area under the curve [AUC], 0.817) and a cutoff of <139.3 for MNES and a sensitivity of 70.4% with a specificity of 76.3% (AUC, 0.752) and a cutoff of <150.9 for MNEC.
全自动血液学细胞分析仪库尔特LH 750（美国加利福尼亚州布雷亚市贝克曼库尔特公司）采用体积、电导率和散射光三种测量方法相结合来识别细胞，但它可以利用这些参数来评估细胞的形态学变化。对54例骨髓增生异常综合征（MDS）患者、18例慢性粒单核细胞白血病（CMML）患者和59例健康对照者的中性粒细胞平均细胞体积（MNEV）、平均细胞电导率（MNEC）和平均细胞散射光（MNES）进行了评估。包括所有亚型（世界卫生组织分类）的MDS组以及CMML患者的MNES和MNEC均显著低于对照组。MDS和CMML中的MNES与显微镜观察评估的细胞质颗粒减少情况相关性良好（P = 0.01）。在本研究中，MNES和MNEC作为中性粒细胞发育异常评估的筛查参数，MNES的敏感性为71.8%，特异性为86.4%（曲线下面积[AUC]，0.817），截断值<139.3；MNEC的敏感性为70.4%，特异性为76.3%（AUC，0.752），截断值<150.9。
